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Neonatal genistein exposure disrupts ovarian and uterine development in the mouse by inhibiting cellular

Guoyun Wu1, Quanwei Wei1, Debing Yu1

  • 1College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.

The Journal of Reproduction and Development
|October 19, 2018
PubMed

Insights

Neonatal exposure to genistein from soy formula disrupts ovarian and uterine development in mice, potentially impacting fertility and reproductive health. Further research into isoflavone-rich diets is recommended.

Area of Science:

  • Reproductive Biology
  • Developmental Toxicology
  • Endocrinology

Background:

  • Soy-based formulas are rich in genistein, an isoflavone with estrogenic and tyrosine kinase inhibitory properties.
  • Genistein's effects on cellular proliferation and development are known, but its long-term impact on ovarian and uterine development requires further investigation.

Purpose of the Study:

  • To investigate the acute and chronic effects of neonatal genistein exposure on ovarian and uterine development in mice.

Main Methods:

  • Neonatal mice (postnatal day 1) received daily subcutaneous injections of genistein (100 mg/kg) for 10 days.
  • Ovaries and uteri were collected on days 10, 21, and 90 for histological evaluation.
  • Proliferating cell activity was assessed using the Ki67 marker.

Main Results:

  • Genistein induced multi-oocyte follicles and delayed primordial follicle assembly in ovaries.
  • Uterine cavity and wall areas were significantly enlarged, with disrupted stroma-myometrium regularity.
  • Genistein inhibited proliferative activity (reduced Ki67-positive nuclei) in ovarian and uterine tissues.
  • Adult mice exposed to neonatal genistein showed ovarian abnormalities, including lack of corpora lutea, cystic follicles, and theca hypertrophy.

Conclusions:

  • Neonatal genistein exposure adversely affects ovarian and uterine development in mice, leading to reproductive abnormalities.
  • The findings suggest a need for closer examination of isoflavone-rich diets, including soy-based infant formulas and livestock feed, due to potential health implications.
Keywords:
GenisteinKi67MorphologyOvaryUterus

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