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Related Concept Videos

Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

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Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
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Master Transcription Regulators02:23

Master Transcription Regulators

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Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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Transcription01:10

Transcription

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Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
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Feedback Inhibition00:46

Feedback Inhibition

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Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
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Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

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The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
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Transcription Factors02:16

Transcription Factors

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Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Related Experiment Video

Updated: Feb 3, 2026

An In Vitro Enzymatic Assay to Measure Transcription Inhibition by GalliumIII and H3 5,10,15-trispentafluorophenylcorroles
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A Novel Dnmt3a1 Transcript Inhibits Adipogenesis.

Bahareldin A Abdalla1,2,3, Zhenhui Li1,2,3, Hongjia Ouyang1,2,3

  • 1Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, China.

Frontiers in Physiology
|October 19, 2018
PubMed
Summary

Researchers identified a novel DNA (cytosine-5)-methyltransferase 3a (Dnmt3a) transcript, Dnmt3a1, in chickens. This new transcript plays a role in regulating adipogenesis by influencing cell proliferation and differentiation.

Keywords:
Dnmt3aDnmt3a1 transcriptagingearly differentiationexpressionhigh-fat dietpreadipocytes proliferation

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Area of Science:

  • Molecular Biology
  • Epigenetics
  • Animal Science

Background:

  • DNA (cytosine-5)-methyltransferase 3a (Dnmt3a) is crucial for DNA methylation.
  • While mammalian Dnmt3a variants are known, avian transcripts remained uncharacterized.
  • Adipogenesis regulation is vital for metabolic health and requires understanding key molecular players.

Purpose of the Study:

  • To identify and characterize novel Dnmt3a transcripts in chickens.
  • To investigate the role of a newly discovered transcript, Dnmt3a1, in adipogenesis.
  • To explore Dnmt3a1's influence on preadipocyte proliferation and differentiation.

Main Methods:

  • Cloning, sequencing, and transcript detection of Dnmt3a in chicken tissues.
  • Expression analysis of Dnmt3a1 under various physiological conditions.
  • Overexpression and knockdown experiments of Dnmt3a1 in preadipocytes.

Main Results:

  • A novel chicken Dnmt3a transcript, Dnmt3a1, lacking 23 amino acids, was identified.
  • Dnmt3a1 is ubiquitously expressed, highly in adipose tissue, and its expression varies with age, fasting, and diet.
  • Dnmt3a1 overexpression inhibited proliferation and induced cell-cycle arrest, while knockdown promoted proliferation; Dnmt3a1 also suppressed adipogenic markers.

Conclusions:

  • A novel chicken Dnmt3a transcript, Dnmt3a1, was discovered and characterized.
  • Dnmt3a1 significantly impacts preadipocyte proliferation and cell-cycle progression.
  • Dnmt3a1 plays a regulatory role in the early stages of adipocyte differentiation.