Externally applied adenosine-5'-triphosphate causes inositol triphosphate accumulation in cultured chick myotubes

In striated muscle, adenosine-5'-triphosphate (ATP) potentiates the responses to acetylcholine. The underlying biochemical events are unknown. Here we report that ATP, externally applied to chick myotubes, induces a rapid, dose-dependent accumulation of intracellular inositol triphosphate which is correlated with a decrease in phosphatidyl 4,5-bisphosphate. Adenosine-5'-diphosphate, adenosine-5'-monophosphate and adenosine are less potent while beta, gamma-imido ATP is equipotent motoneurons and/or skeletal muscle controls the activation of a polyphosphoinositide phosphodiesterase via a cell membrane P2-purinoceptor, thus modulating skeletal muscle responses to transmitter release.