A coumarin based fluorescent probe for rapidly distinguishing of hypochlorite and copper (II) ion in organisms

A dual-functional fluorescent probe based on coumarin fluorophore for monitoring ClO^- and Cu²⁺ was synthesized and characterized. The identification mechanisms for ClO^- and Cu²⁺ induced different colorimetric and ratiometric changes. ClO^- caused a colorimetric change from yellow to colorless and 120 nm blue-shifted emission spectra from red to blue. Besides, Cu²⁺ induced a remarkable fluorescence quenching behavior and 36 nm blue-shifted absorption spectra accompanied by the color change from yellow to luminous yellow. As expected, Probe 1 displayed excellent selectivity and sensitivity for detecting ClO^- and Cu²⁺ over other competing ions in their respective systems. The limits of detection for ClO^- and Cu²⁺ were 24.62 nM and 0.39 nM, respectively. The recognition mechanisms were proved by ¹H NMR, mass spectrum and theoretical calculations. More importantly, the reversibility of Cu²⁺ could be applicable to rapid quantification for ClO^-/Cu²⁺. Thus, the strategy for sensing ClO^-/Cu²⁺ was carried out, which revealed broad applications of Probe 1 in multiple actual water and biology.