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Unveiling gibberellin-responsive coding and long noncoding RNAs in maize.

Yijun Wang1,2, Yali Wang3,4, Jia Zhao3,4

  • 1Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Agricultural College of Yangzhou University, Yangzhou, 225009, China. wyj@yzu.edu.cn.

Plant Molecular Biology
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Summary
This summary is machine-generated.

Gibberellin (GA) hormone stimulation alters gene expression in maize, revealing novel coding and long noncoding RNAs (lncRNAs). This study details GA-responsive lncRNAs and their targets, enhancing understanding of plant growth regulation.

Keywords:
Advanced backcross populationCoding RNAGibberellinLong noncoding RNAMaize (Zea mays L.)

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Area of Science:

  • Plant Biology
  • Molecular Biology
  • Genomics

Background:

  • Plant hormone gibberellin (GA) regulates critical biological processes in plants.
  • Understanding the maize transcriptome's response to GA is crucial for deciphering plant growth mechanisms.
  • Previous studies have not fully elucidated the transcriptomic dynamics following GA application in maize.

Purpose of the Study:

  • To investigate the coding and long noncoding RNA (lncRNA) profiles in maize after gibberellin (GA3) stimulation.
  • To identify differentially expressed genes and lncRNAs in both normal height and GA-sensitive dwarf maize plants.
  • To uncover potential targets of GA-responsive lncRNAs involved in plant development.

Main Methods:

  • Transcriptomic analysis using RNA sequencing on ribosomal-depleted libraries from maize plants treated with varying GA3 concentrations.
  • Comparative analysis of gene expression in normal height and GA-sensitive dwarf maize.
  • Identification and characterization of differentially expressed coding RNAs and lncRNAs.

Main Results:

  • Significant differential expression of transcripts encoding GA biosynthetic and metabolic enzymes (KS, GA20ox, GA3ox, GA2ox) upon GA3 treatment.
  • Identification of 78 shared protein-coding transcripts and several shared GA-responsive lncRNAs between normal and dwarf maize lines.
  • Discovery of GA-responsive lncRNAs, some corresponding to known miRNA precursors (e.g., zma-miR528a/b), and identification of targets like Lazy plant1.

Conclusions:

  • GA3 stimulation significantly impacts the maize transcriptome, affecting both coding and noncoding RNAs.
  • Shared GA-responsive lncRNAs and protein-coding transcripts suggest conserved regulatory mechanisms in different maize genotypes.
  • This research provides insights into GA-triggered transcriptomic changes and identifies potential regulators of GA-mediated plant development.