Related Experiment Video
Updated: Feb 3, 2026

Performing Data Mining And Integrative Analysis Of Biomarker in Breast Cancer Using Multiple Publicly Accessible Databases
Published on: May 17, 2019
Identification of genes associated with survival of breast cancer patients
Min Liu1, Siying Zhou2, Jinyan Wang3
1Department of Clinical Laboratory, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, 210009, China.
Background:
We aimed to investigate the potential of microRNA expression profiles to predict survival in breast cancer.
Methods:
MicroRNA and mRNA expression data of breast cancer were downloaded from The Cancer Genome Atlas. LASSO regression was used to identify microRNAs signature predicting survival of breast cancer patients. Transfection experiment was conducted to explore the influence of microRNAs on their potential targets.
Results:
We identified 56 differentially expressed microRNAs in breast cancer tissues compared to adjacent normal tissues. 10 microRNAs with non-zero coefficient were selected from the 56 microRNAs using LASSO Cox regression. After predicting the targets for the 10 microRNAs, we further obtained 155 targets that were associated with overall survival of breast cancer patients. Spearman's correlation analysis found that the expression of SCUBE2, SCRN3, YTHDF3, ITFG1, ITPRIPL2, and JAK1 was an inversely correlated with their microRNAs. Transfection experiment showed that YTHDF3 was down-regulated in cells transfected with miR-106b-5p mimics compared with those transfected with negative control of mimics (fold change 4.21; P < 0.01).
Conclusions:
In conclusion, we identified a 10-miRNA signature associated with prognosis of breast cancer patients. The expression of YTHDF3 was down-regulated by miR-106b-5p.
More Related Videos
06:06High-Throughput Dissociation and Orthotopic Implantation of Breast Cancer Patient-Derived Xenografts
Published on: December 20, 2024
11:34Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers
Published on: December 5, 2017
Related Concept Videos
Cancer Survival Analysis
Cancer-Critical Genes II: Tumor Suppressor Genes
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Survival Curves
The Kaplan-Meier estimator is the most common method for constructing survival curves. This...
Survival Tree
Building a Survival Tree
Constructing a...
Cancer-Critical Genes I: Proto-oncogenes
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Gene Flow