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Performing Data Mining And Integrative Analysis Of Biomarker in Breast Cancer Using Multiple Publicly Accessible Databases
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mRNA Expression of CDK2AP1 in Human Breast Cancer: Correlation with Clinical and Pathological Parameters

Ritika Gera1, Leon Mokbel1, Wen G Jiang2

  • 1The London Breast Institute, The Princess Grace Hospital, London, U.K.

Cancer Genomics & Proteomics
|October 22, 2018
PubMed
Abstract

Insights

Cyclin-dependent kinase 2-associated protein 1 (CDK2AP1) mRNA expression is higher in non-cancerous breast tissue and correlates with lower tumor stage. This suggests CDK2AP1 may function as a tumor suppressor in breast cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Expression Analysis

Background:

  • Cyclin-dependent kinase 2-associated protein 1 (CDK2AP1) is a protein with known interactions with cell cycle regulators and microRNAs.
  • Its potential role in breast cancer, particularly its tumor-suppressive capacity, requires further investigation.

Purpose of the Study:

  • To investigate the messenger RNA (mRNA) expression levels of CDK2AP1 in breast cancer tissues.
  • To determine if CDK2AP1 expression correlates with tumor stage, clinical parameters, and patient survival.

Main Methods:

  • Quantitative real-time polymerase chain reaction (RT-PCR) was used to measure CDK2AP1 mRNA levels in 134 breast cancer and adjacent non-cancerous tissue samples.
  • Expression levels were analyzed in relation to TNM staging, estrogen receptor status, tumor grade, tumor type, and 10-year patient survival data.

Main Results:

  • CDK2AP1 mRNA expression was significantly higher in non-cancerous breast tissue compared to breast cancer tissue (38-fold difference).
  • Higher CDK2AP1 expression correlated with lower TNM staging (TNM1 vs. TNM3/TNM4, p=0.016).
  • No significant association was found between CDK2AP1 expression and overall survival, estrogen receptor status, tumor grade, or tumor type.

Conclusions:

  • This study provides the first long-term follow-up analysis of CDK2AP1 mRNA expression in human breast cancer.
  • The observed inverse correlation between high CDK2AP1 mRNA levels and lower TNM classification supports its potential role as a tumor suppressor in breast cancer.
Keywords:
Breast cancerCDK2AP1breast cancer specimenhuman breast cancertumour suppressor roletumour-suppressive function

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