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Absence of toll-like receptor 9 Pro99Leu polymorphism in cervical cancer

Alex Chauhan1, Nilesh Pandey1, Nitin Raithatha2

  • 1P D Patel Institute of Applied Sciences, Charotar University of Science and Technology (CHARUSAT), Changa, India.

F1000Research
|October 23, 2018
PubMed

Insights

This study investigated the Toll-like receptor 9 (TLR9) Pro99Leu polymorphism

Area of Science:

  • Immunogenetics
  • Oncology
  • Molecular Biology

Background:

  • Toll-like receptor 9 (TLR9) recognizes pathogen-associated molecular patterns like CpG DNA, crucial for antiviral immunity.
  • Genetic variations in TLR9 may impact pathogen recognition and immune response.
  • Cervical cancer involves complex interactions between host immunity and oncogenic factors.

Purpose of the Study:

  • To investigate the association between the rare TLR9 Pro99Leu (C296T, rs5743844) gene polymorphism and cervical cancer susceptibility in Indian women.
  • To determine if this specific TLR9 polymorphism influences the risk of developing cervical cancer.

Main Methods:

  • Genotyping of the TLR9 Pro99Leu polymorphism using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP).
  • Study included 110 cervical cancer patients and 141 healthy controls from an Indian population.
  • Analysis of genotype and allele frequencies for the polymorphism.

Main Results:

  • The CC genotype was the only one detected in both cervical cancer patients (frequency 1.0) and healthy controls (frequency 1.0).
  • The C allele was present with a frequency of 1.0 in both groups, while the T allele was absent (frequency 0.0).
  • No nucleotide variations (CT or TT genotypes) were observed in any study participants.

Conclusions:

  • The TLR9 C296T/Pro99Leu polymorphism is not associated with cervical cancer susceptibility in the studied Indian population.
  • The findings support the rare nature and low minor allele frequency (MAF) of this polymorphism.
  • Further research may be needed to explore other TLR9 variants in relation to cervical cancer.
Keywords:
Cervical cancerGenotypic frequencyPolymorphismSusceptibilityTLR9

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