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Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models
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Highly active fluorogenic oxidase-mimicking NiO nanozymes.

Dai Li1, Biwu Liu, Po-Jung Jimmy Huang

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|October 23, 2018
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Nickel oxide (NiO) nanoparticles function as oxidase nanozymes, catalyzing reactions without hydrogen peroxide. This discovery offers new possibilities for biosensors and intracellular imaging applications.

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Area of Science:

  • Nanotechnology
  • Biochemistry
  • Materials Science

Background:

  • Oxidase-mimicking nanozymes are highly sought after for their ability to perform oxidation reactions without requiring hydrogen peroxide (H2O2).
  • However, examples of such nanozymes, particularly those capable of catalyzing the oxidation of fluorogenic substrates, are scarce.
  • This limitation hinders the development of advanced biosensors and imaging techniques.

Purpose of the Study:

  • To identify and characterize novel nanozymes with oxidase-like activity that do not require H2O2.
  • To evaluate the potential of these nanozymes in catalyzing the oxidation of fluorogenic substrates.
  • To address the existing gap in nanozyme applications for biosensing and intracellular imaging.

Main Methods:

  • Synthesis and characterization of Nickel Oxide (NiO) nanoparticles.
  • Assay development to evaluate nanozyme activity at physiological pH.
  • Comparative analysis of NiO nanoparticle activity against established nanozymes like nanoceria using fluorogenic substrates (Amplex Red).

Main Results:

  • Nickel Oxide (NiO) nanoparticles demonstrated significant oxidase-like nanozyme activity at physiological pH.
  • NiO nanoparticles effectively catalyzed the oxidation of the fluorogenic substrate Amplex Red.
  • The catalytic activity of NiO nanoparticles was substantially higher compared to commonly used nanoceria.

Conclusions:

  • NiO nanoparticles represent a novel and effective oxidase nanozyme, overcoming the need for H2O2.
  • This finding provides a valuable new tool for developing advanced biosensors and for intracellular imaging.
  • The discovery addresses a critical need in the nanozyme field, expanding its potential applications.