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Updated: Feb 3, 2026

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
Published on: February 3, 2021
Homogeneous enzyme-free and entropy-driven isothermal fluorescent assay for nucleic acids based on a dual-signal
Erhu Xiong1, Deshuai Zhen1, Ling Jiang1
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China. ljiang630@163.com.
Abstract:
Herein, we developed a homogeneous enzyme-free, ultrasensitive and isothermal fluorescent biosensor for nucleic acid detection with dual-signal output to enhance the reaction rate and sensitivity by combining an entropy-driven catalysis strategy with a catalytic hairpin assembly reaction.
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