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Updated: Feb 3, 2026

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Construction of a recombinant eukaryotic expression vector containing DNM3 gene and its expression in colon cancer

Liang Jiang1, Qi-Lian Liang2, Wei-Ming Liang1

  • 1Interventional Ward, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China, 13600389991@139.com.

Oncotargets and Therapy
|October 24, 2018
PubMed
Abstract

Insights

Dynamin 3 (DNM3) overexpression in colon cancer cells suppressed proliferation, invasion, and migration. DNM3 may weaken cancer malignancy by downregulating matrix metalloproteinase (MMP)-2 and MMP-9 expression.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Cell Biology

Background:

  • Dynamin 3 (DNM3) is a GTPase implicated in malignancies, but its precise molecular mechanisms are not fully understood.
  • Existing research on DNM3 in cancer is largely observational, necessitating further mechanistic studies.

Purpose of the Study:

  • To investigate the role and molecular mechanism of Dynamin 3 (DNM3) in colon cancer cell lines.
  • To determine the effect of DNM3 overexpression on colon cancer cell proliferation, migration, and invasion.

Main Methods:

  • Constructed a DNM3 recombinant eukaryotic expression vector and transfected it into SW620 and LoVo colon cancer cells.
  • Analyzed DNM3 mRNA and protein expression using quantitative real-time PCR and Western blot.
  • Assessed cell proliferation, migration, and invasion, and detected matrix metalloproteinase (MMP)-2 and MMP-9 protein levels.

Main Results:

  • Successfully established stable DNM3-overexpressing colon cancer cell lines (SW620 and LoVo).
  • DNM3 overexpression significantly inhibited cell proliferation, invasion, and migration compared to control groups (P<0.001).
  • DNM3 significantly downregulated the protein expression of MMP-2 and MMP-9.

Conclusions:

  • Dynamin 3 (DNM3) overexpression weakens the malignant behavior of colon cancer cells.
  • DNM3 may inhibit colon cancer invasion and migration by regulating MMP-2 and MMP-9 expression.
Keywords:
colon cancerdynamin 3invasionmigrationproliferation

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