Automated multicomponent phospholipid analysis using ³¹P NMR spectroscopy: example of vegetable lecithin and krill...

Nuclear magnetic resonance (NMR) spectroscopy is widely applied in the field of metabolomics due to its quantitative nature and the reproducibility of data generated. However, one of the main challenges in routine NMR analysis is to obtain valuable information from large datasets of raw data in a high-throughput, automatic, and reproducible manner. In this study, a method to automatically annotate and quantify 12 phospholipids (PLs) in vegetable lecithin (soy, sunflower, rape) and krill oil is introduced. Automated routines were written in MATLAB environment for quantification of phosphatidylcholine (PC), phosphatidylinositol (PI), lyso-phosphatidylcholine (LPC), phosphatidylserine (PS), phosphatidylethanolamine (PE), diphosphatidylglycerol or cardiolipin (DPG), phosphatidylglycerol (PG), and lyso-phosphatidylethanolamine (LPE) in lecithin and of PC, PC-ether, LPC, PE, N-acyl phosphatidylethanolamine (APE), and LPE in krill oil matrix. The routine includes NMR spectra import, extraction of data points, peaking of local minima and local maxima in the data, integration, quantitation against internal standard, reporting of results as Word file, and their importing in our internal database. Our extensive studies on a representative set of more than 1000 lecithin (soy, rape, sunflower) and krill samples showed that the routine can automatically and accurately calculate the concentrations of all PLs. No systematic or proportional differences between automated and manual evaluation were detected. The developed automated program produces accurate results and requires less than 5 s for each analysis. This tool is already used in high-throughput PL analysis of krill and lecithin and will be adjusted to other matrices (egg, milk, chocolate, etc.) as well.