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Updated: Sep 11, 2025
09:24
Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT
Published on: December 18, 2020
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HEteronuclear Referencing for METRologic Isotope Calibration (HERMETRIC).
Bernd W K Diehl1,2, Jakob V Waldthausen1,3, Yulia Monakhova1,3,4
1Spectral Service AG, Cologne, Germany.
Magnetic Resonance in Chemistry : MRC
|August 18, 2025
Summary
Heteronuclear referencing for metrologic isotope calibration (HERMETRIC) offers a new primary method for quantitative NMR (qNMR) spectroscopy. This approach enables direct, uncalibrated substance amount determination and traceability for all nuclei to a universal standard.
Area of Science:
- Analytical Chemistry
- Spectroscopy
- Metrology
Background:
- Quantitative NMR (qNMR) traditionally relies on homonuclear methods.
- Established quantitative techniques like weighing and chromatography require external calibration.
- There is a need for primary methods in qNMR for direct substance amount determination.
Purpose of the Study:
- Introduce heteronuclear referencing for metrologic isotope calibration (HERMETRIC) as a novel qNMR approach.
- Establish a metrologically sound foundation for qNMR beyond homonuclear techniques.
- Enable direct traceability of all active nuclei to a universal qNMR primary standard.
Main Methods:
- Development and application of the HERMETRIC concept for qNMR.
- Demonstration of qNMR as a primary method for absolute substance amount determination.
- Comparison with traditional quantitative methods (weighing, titration, chromatography, complexometry).
Main Results:
- HERMETRIC enables direct quantitative NMR (qNMR) without external calibration.
- Absolute amounts of substance can be determined directly using qNMR.
- Heteronuclear quantification provides traceability for all active nuclei to a universal qNMR standard.
Conclusions:
- qNMR, utilizing HERMETRIC, can function as a primary method for accurate substance quantification.
- The HERMETRIC concept expands the capabilities of qNMR in analytical chemistry.
- This approach facilitates the integration of qNMR into pharmacopoeias and official standards.
Keywords:
analytical principlescomplexometryheteronuclear quantitative NMR spectroscopyisotopic analysismethods comparisontitrationweighingMore Related Videos
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