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Updated: Feb 3, 2026

In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers
Published on: July 28, 2018
Proteins on Supported Lipid Bilayers Diffusing around Proteins Fixed on Acrylate Anchors
Bianca Buchegger1, Johannes Kreutzer1, Markus Axmann2
1Institute of Applied Physics , Johannes Kepler University Linz , Altenberger Straße 69 , 4040 Linz , Austria.
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Mobility of proteins and lipids plays a major role in physiological processes. Platforms which were developed to study protein interaction between immobilized and mobile proteins suffer from shortcomings such as fluorescence quenching or complicated fabrication methods. Here we report a versatile platform comprising immobilized histidine-tagged proteins and biotinylated proteins in a mobile phase. Importantly, multiphoton photolithography was used for easy and fast fabrication of the platform and allows, in principle, extension of its application to three dimensions. The platform, which is made up of functionalized polymer structures embedded in a mobile lipid bilayer, shows low background fluorescence and allows for mobility of arbitrary proteins.
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