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Virus-inactivating effect of D-isoascorbic acid.

A Murata, M Kawasaki, H Motomatsu

Journal of Nutritional Science and Vitaminology

|December 1, 1986

Summary

D-isoascorbic acid inactivates various bacterial viruses (phages). This antiviral activity, mediated by hydroxyl radicals, is independent of stereoisomerism, similar to L-ascorbic acid.

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Area of Science:

Virology
Biochemistry
Antiviral Research

Background:

L-ascorbic acid (Vitamin C) is known for its biological activities, including potential antiviral effects.
D-isoascorbic acid is a stereoisomer of L-ascorbic acid, differing in configuration at carbon 5.
The antiviral properties of D-isoascorbic acid have not been extensively studied.

Purpose of the Study:

To investigate the effect of D-isoascorbic acid on bacterial viruses (phages).
To elucidate the mechanism of virus inactivation by D-isoascorbic acid.
To compare the antiviral activity of D-isoascorbic acid with that of L-ascorbic acid.

Main Methods:

Bacterial viruses (phages) were used as model systems to test inactivation by D-isoascorbic acid.
Reaction conditions were manipulated, including oxygen/nitrogen bubbling and addition of H2O2, metal ions, reducing agents, chelating agents, and radical scavengers.
Specific radical scavengers like superoxide dismutase and catalase were employed to identify the reactive species involved.

Main Results:

D-isoascorbic acid demonstrated an inactivating effect on all tested phages.
Phage inactivation was enhanced by oxygen, H2O2, and transition metal ions.
Inactivation was inhibited by reducing agents, chelating agents, radical scavengers, and nitrogen bubbling.
Hydroxyl radicals (OH.) were identified as the primary species responsible for inactivation.

Conclusions:

D-isoascorbic acid possesses significant phage-inactivating activity.
The mechanism involves reactive oxygen species, particularly hydroxyl radicals.
Antiviral activity is independent of the stereoisomerism of ascorbic acid, suggesting a conserved mechanism for both isomers.

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