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Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases
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Generation of Rabbit Models by Gene Editing Nucleases
Dongshan Yang1, Jie Xu1, Y Eugene Chen2
1Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center, Ann Arbor, MI, USA.
Methods in Molecular Biology (Clifton, N.J.)
|October 25, 2018
Summary
Gene editing technologies like CRISPR/Cas9 now enable efficient gene targeting in rabbits, overcoming previous limitations in creating genetically engineered models for research.
Area of Science:
- Animal genetics
- Molecular biology
- Biotechnology
Background:
- Rabbit gene targeting lagged due to challenges with pluripotent stem cells and somatic cell nuclear transfer.
- Limited development and application of genetically engineered rabbit models hindered research.
Purpose of the Study:
- To present a comprehensive protocol for rabbit genome editing.
- To detail the application of gene editing nucleases, particularly CRISPR/Cas9, for creating genetically modified rabbits.
Main Methods:
- Utilizing advanced gene editing nucleases such as CRISPR/Cas9.
- Developing a step-by-step protocol for efficient genome modification in rabbits.
Main Results:
- High success rates in producing gene targeting (knockout and knockin) rabbits.
- Overcoming previous technical barriers in rabbit genetic engineering.
Conclusions:
- Gene editing nucleases have revolutionized the creation of genetically engineered rabbits.
- This protocol facilitates the development and application of rabbit models for scientific research.
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