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Related Experiment Video

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Endothelial-Specific Cre Mouse Models.

Sophie Payne1, Sarah De Val1,2, Alice Neal2

  • 1From the Ludwig Institute for Cancer Research Ltd, Nuffield Department of Medicine (S.P., S.D.V.),University of Oxford, United Kingdom.

Arteriosclerosis, Thrombosis, and Vascular Biology
|October 26, 2018
PubMed
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Cre mouse models are vital for vascular biology research, enabling gene manipulation in endothelial cells. Careful selection and analysis are crucial, as Cre activity varies, impacting experimental outcomes.

Keywords:
endothelial cellsgene expressionmicephenotyperecombinases

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Area of Science:

  • Vascular Biology
  • Genetics
  • Mouse Models

Background:

  • Cre and inducible Cre mouse models are essential tools in vascular biology.
  • These models allow for temporal and spatial control of gene expression in endothelial cells.
  • However, variations in Cre activity and expression can complicate experimental results.

Purpose of the Study:

  • To summarize Cre recombinase activity in widely used endothelial-specific Cre and Cre/ERT2 mouse models.
  • To provide a resource for researchers to aid in experimental design.
  • To disambiguate previous findings related to Cre mouse models in vascular biology.

Main Methods:

  • Review and summarization of existing literature on endothelial-specific Cre and Cre/ERT2 mouse models.
  • Analysis of reported Cre recombinase activity patterns.
  • Comparison of different Cre models and their expression characteristics.

Main Results:

  • Many endothelial-specific Cre models exhibit divergent activity patterns.
  • Ectopic or inconsistent Cre expression can significantly alter experimental phenotypes.
  • A comprehensive summary of Cre activity in key endothelial models is presented.

Conclusions:

  • Researchers must exercise caution when selecting Cre mouse models for endothelial studies.
  • Understanding the specific activity patterns of Cre and inducible Cre is critical for accurate interpretation of results.
  • This review serves as a guide for appropriate experimental design using endothelial Cre models.