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Updated: Feb 3, 2026

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
Published on: October 18, 2010
Rapid Sample Preparation and LAMP for Phytoplasma Detection
1Fera, The National Agri-Food Innovation Campus, Sand Hutton, York, UK. jennifer.hodgetts@fera.co.uk.
Abstract:
Loop-mediated isothermal AMPlification (LAMP) allows the rapid detection of pathogens by polymerase-mediated amplification of target nucleic acid sequences at a single incubation temperature. LAMP can be combined with very simple sample preparation/crude DNA extraction protocols, allowing the method to be used away from the laboratory for in-field detection. Equally, these benefits can also be leveraged to provide a rapid method suited to high-throughput diagnostic laboratories. In this chapter we described a crude DNA extraction protocol suitable for use in the field and provide a protocol for real-time detection using LAMP.
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