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Related Experiment Videos

DNA barcoding for biosecurity: case studies from the UK plant protection program.

Jennifer Hodgetts1,1, Jozef C Ostojá-Starzewski1,1, Thomas Prior1,1

  • 1Fera, The National Agri-Food Innovation Campus, Sand Hutton, York, YO41 1LZ, United Kingdom.

Genome
|October 30, 2016
PubMed
Summary

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DNA barcoding aids plant health diagnostics by identifying invertebrate pests, including regulated pests like Bursephalenchus xylophilus and its vectors. Case studies show successful applications and highlight limitations for broader use in diagnostic labs.

Area of Science:

  • Plant pathology
  • Entomology
  • Molecular biology

Background:

  • DNA barcoding is a molecular tool for species identification.
  • Its adoption in routine diagnostic laboratories has been gradual.
  • Defra plant health laboratories utilize DNA barcoding for invertebrate pest identification.

Purpose of the Study:

  • To discuss the application of DNA barcoding in plant health diagnostics.
  • To present case studies of DNA barcoding use in identifying invertebrate pests.
  • To identify limitations and potential for expanded use of DNA barcoding.

Main Methods:

  • DNA barcoding for species identification.
  • Case study analysis of pest identification in imported goods and agricultural settings.
Keywords:
DNA barcodingcodage à barres de l’ADNdiagnosticsdiagnostiquesinvertebrateinvertébrésorganisme nuisible réglementéplant healthregulated quarantine pestsanté des végétaux

Related Experiment Videos

  • Generation of reference sequences for psyllids.

    • Main Results:

    • DNA barcoding successfully identified regulated pests (Bursephalenchus xylophilus) and vectors (Monochamus alternatus) in imported dining chairs.
    • Several longhorn beetle species were identified in various wooden products.
    • An outbreak of Meloidogyne fallax was detected in Allium ampeloprasum and weeds.
    • Reference sequences for UK native psyllids were generated.

    Conclusions:

    • DNA barcoding is effective for identifying high-risk invertebrate pests in trade and agricultural settings.
    • Case studies demonstrate practical applications but also reveal limitations for widespread diagnostic use.
    • The generated reference sequences support monitoring efforts, such as for biological control agents.