Related Experiment Video

Updated: Feb 3, 2026

Optimizing Isolation and Purification of Murine Glomerular Mesangial Cells
04:46

Optimizing Isolation and Purification of Murine Glomerular Mesangial Cells

Published on: March 7, 2025

980

CTRP3 inhibits high glucose-induced human glomerular mesangial cell dysfunction

Tian-Ying Hu1, La-Mei Li2, Yan-Zi Pan3

  • 1Department of Endocrinology, Weinan Central Hospital, Weinan, Shaanxi, China.

Journal of Cellular Biochemistry
|October 27, 2018
PubMed

Insights

C1q/tumour necrosis factor-related protein-3 (CTRP3) levels decrease in diabetes. CTRP3 protects against high glucose-induced kidney cell damage by inhibiting proliferation and extracellular matrix buildup via the JAK2/STAT3 pathway.

Area of Science:

  • Endocrinology
  • Nephrology
  • Molecular Biology

Background:

  • C1q/tumour necrosis factor-related protein-3 (CTRP3) levels are reduced in obesity and diabetes.
  • The role of CTRP3 in diabetic nephropathy is not well understood.

Purpose of the Study:

  • To investigate the effect of CTRP3 on human glomerular mesangial cells (MCs) under high glucose conditions.
  • To explore the molecular mechanisms underlying CTRP3's action in diabetic nephropathy.

Main Methods:

  • Assessed CTRP3 expression in MCs stimulated with high glucose.
  • Overexpressed CTRP3 in MCs to evaluate its impact on proliferation, reactive oxygen species (ROS), and extracellular matrix (ECM) production.
  • Investigated the involvement of the Janus kinase 2/signal transducers and activators of transcription 3 (JAK2/STAT3) pathway.

Main Results:

  • High glucose significantly decreased CTRP3 expression in MCs.
  • CTRP3 overexpression inhibited MC proliferation, ROS levels, and ECM accumulation in high glucose-treated MCs.
  • CTRP3 overexpression suppressed the activation of the JAK2/STAT3 signaling pathway.

Conclusions:

  • CTRP3 attenuates high glucose-induced MC proliferation and ECM production.
  • CTRP3 exerts its protective effects by inactivating the JAK2/STAT3 signaling pathway.
  • CTRP3 represents a potential therapeutic target for diabetic nephropathy.
Keywords:
CTRP3JAK2/STAT3 pathwaydiabetic nephropathyextracellular matrix (ECM)

More Related Videos

Isolation of Conditionally Immortalized Mouse Glomerular Endothelial Cells with Fluorescent Mitochondria11:49

Isolation of Conditionally Immortalized Mouse Glomerular Endothelial Cells with Fluorescent Mitochondria

Published on: September 13, 2022

2.7K
Derivation of Highly Purified Cardiomyocytes from Human Induced Pluripotent Stem Cells Using Small Molecule-modulated Differentiation and Subsequent Glucose Starvation11:53

Derivation of Highly Purified Cardiomyocytes from Human Induced Pluripotent Stem Cells Using Small Molecule-modulated Differentiation and Subsequent Glucose Starvation

Published on: March 18, 2015

19.6K

Related Concept Videos

Feedback Inhibition00:46

Feedback Inhibition

Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
57.1K
Glomerular Filtration01:15

Glomerular Filtration

The filtration membrane in the renal system is a highly specialized structure essential for filtering blood. It consists of glomerular capillaries and podocytes, forming a selective barrier that permits the passage of water and small solutes while restricting most plasma proteins and blood cells.
Components of the Filtration Membrane
The filtration process involves three key layers: the glomerular endothelial cells, the basement membrane, and the podocyte-formed filtration slits.
5.4K
Glucose Homeostasis: Regulation of Blood Glucose01:02

Glucose Homeostasis: Regulation of Blood Glucose

Carbohydrates consumed through foods are converted into glucose, a crucial energy source for the body. In the prandial state, high blood glucose levels stimulate the secretion of insulin from the pancreas. Insulin inhibits hepatic glucose production and stimulates glucose uptake and metabolism by muscle and adipose tissue. The excess glucose is converted into glycogen and stored in the liver and muscles.
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
4.1K
Glucose Transporters01:27

Glucose Transporters

Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
27.5K
Glomerular Filtration Rate and its Regulation01:28

Glomerular Filtration Rate and its Regulation

The Glomerular Filtration Rate (GFR) is a measure of kidney function, reflecting the volume of filtrate formed per minute in the kidneys. On average, GFR is approximately 125 mL/min in males and 105 mL/min in females. Maintaining a relatively constant GFR is essential for the kidneys to effectively regulate body fluid homeostasis and maintain extracellular stability.
GFR regulation involves two primary intrinsic controls: the myogenic and tubuloglomerular feedback mechanisms.
The myogenic...
5.4K
Glomerular Filtration: Net Filtration Pressure01:26

Glomerular Filtration: Net Filtration Pressure

Glomerular filtration, a key process in the kidneys, is regulated by three main pressures: Glomerular blood hydrostatic pressure (GBHP), Capsular hydrostatic pressure (CHP), and Blood colloid osmotic pressure (BCOP).
GBHP, with an average value of 55 mmHg, promotes filtration by pushing water and solutes through the filtration membrane. This is balanced by two opposing forces: CHP, a "back pressure" exerted against the filtration membrane by fluid already in the capsular space and renal...
8.2K