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Individualized Reconstitution of Human Milk Microbiota: A Feasible Approach in Real-World Settings
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Pasteurization Preserves IL-8 in Human Milk.

Marilyn V Giorgi1, Champa N Codipilly1, Debra Potak1

  • 1Lilling Family Neonatal Research Lab, Division of Neonatal-Perinatal Medicine, Cohen Children's Medical Center, Zucker School of Medicine at Hofstra, Northwell, Feinstein Institute for Medical Research, New York, NY, United States.

Frontiers in Pediatrics
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PubMed
Summary
This summary is machine-generated.

Holder pasteurization of donor human milk preserves interleukin-8 (IL-8) but reduces anti-inflammatory cytokines. This may explain why pasteurized milk offers less protection for premature infants compared to mother's own milk.

Keywords:
cytokinesdonor human milkhuman milkpasteurized donor human milkpremature infants

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Area of Science:

  • Neonatal nutrition
  • Human milk immunology
  • Infant feeding practices

Background:

  • Pasteurized donor human milk is crucial for premature infants when mother's milk is unavailable.
  • The impact of pasteurization on human milk's host defense properties remains incompletely understood.
  • Investigating the effects of Holder pasteurization on cytokine concentrations in human milk is essential.

Purpose of the Study:

  • To quantify and compare anti-inflammatory and pro-inflammatory cytokine concentrations in donor human milk before and after Holder pasteurization.
  • To assess the stability of key cytokines, including IL-8, following heat treatment.

Main Methods:

  • Collected milk samples from 24 mothers of premature infants.
  • Divided samples into two aliquots: one control, one pasteurized at 62.5°C for 30 minutes.
  • Analyzed cytokine concentrations using rapid thawing and batch analyses.

Main Results:

  • Most cytokine concentrations significantly decreased after pasteurization.
  • Interleukin-8 (IL-8), a prevalent cytokine, demonstrated high preservation (89%) post-pasteurization.
  • No correlation found between milk storage duration, infant gestational age, or postnatal age and cytokine levels.

Conclusions:

  • Holder pasteurization preserves or liberates IL-8 in human milk, unlike most other cytokines.
  • The loss of anti-inflammatory cytokines and preservation of IL-8 warrants further evaluation of inflammatory activity in pasteurized milk.
  • These findings may partially explain the reduced host-protective effects of pasteurized donor milk compared to mother's own milk.