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Pancreatic Tissue-Derived Extracellular Matrix Bioink for Printing 3D Cell-Laden Pancreatic Tissue Constructs
Published on: December 13, 2019
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Gallol-derived ECM-mimetic adhesive bioinks exhibiting temporal shear-thinning and stabilization behavior
Mikyung Shin1, Jonathan H Galarraga2, Mi Y Kwon2
1Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), 291 University Road, Yuseong-gu, Daejeon 34141, South Korea; Department of Bioengineering, University of Pennsylvania, 210 South 33rd Street, Philadelphia, PA 19104, USA.
Acta Biomaterialia
|October 27, 2018
Summary
Researchers developed a novel gallol-modified extracellular matrix (ECM) hydrogel ink for 3D bioprinting. This bioink offers tunable properties, immediate gelation, and cytocompatibility, advancing tissue engineering and disease modeling applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Bioprinting Technology
Background:
- 3D bioprinting requires advanced bioinks that mimic the cellular microenvironment and possess suitable printing properties.
- Existing hydrogel bioinks often face limitations in cytocompatibility, viscosity, and stabilization for complex tissue fabrication.
- Developing novel bioinks is crucial for improving the efficacy of tissue repair and disease modeling.
Purpose of the Study:
- To develop a unique gallol-modified extracellular matrix (ECM) hydrogel ink for 3D bioprinting.
- To investigate the ink's properties, including shear-thinning, gelation kinetics, and cytocompatibility.
- To demonstrate the potential of this novel bioink for fabricating cell-laden constructs.
Main Methods:
- Gallol modification of ECM components (hyaluronic acid, gelatin) to create a novel hydrogel ink.
- Characterization of the hydrogel ink's rheological properties, including shear-thinning and immediate gelation.
- Assessment of the ink's cytocompatibility (viability >95%) and printability using extrusion-based 3D printing.
- Evaluation of printed cell-laden constructs over 6 days in culture.
Main Results:
- The gallol-modified ECM hydrogel exhibited rapid gelation via dynamic hydrogen bonds and shear-thinning behavior.
- The ink demonstrated temporal stabilization through auto-oxidation and covalent crosslinking post-printing.
- Extrusion-based 3D printing was successful, yielding cell-laden filaments with high cytocompatibility (~95% viability).
- Printed constructs showed degradation and swelling, correlating with increased cell density and spreading over 6 days.
Conclusions:
- The gallol-modified ECM hydrogel ink presents a versatile platform for 3D bioprinting with tunable properties.
- The ink's unique properties, including shear-thinning, covalent crosslinking, and adhesiveness, enable efficient bioprinting and on-tissue applications.
- This approach offers a significant advancement in designing advanced bioinks for tissue engineering and regenerative medicine.
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