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Related Concept Videos

Storage01:23

Storage

410
A schema is a mental framework that helps individuals organize and interpret information. Schemata, formed from previous experiences, influence how we process new information: how we encode it, the inferences we make, and how we retrieve it. For instance, a schema for what a typical classroom looks like might include desks, a teacher's desk, a whiteboard, and students in such an environment. This expectation helps us quickly understand and navigate new classrooms without needing to analyze...
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Effects of EDTA on End-Point Detection Methods01:18

Effects of EDTA on End-Point Detection Methods

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Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
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ATP Energy Storage and Release01:31

ATP Energy Storage and Release

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ATP is a highly unstable molecule. Unless quickly used to perform work, ATP spontaneously dissociates into ADP and inorganic phosphate (Pi), and the free energy released during this process is lost as heat. The energy released by ATP hydrolysis is used to perform work inside the cell and depends on a strategy called energy coupling. Cells couple the exergonic reaction of ATP hydrolysis with endergonic reactions, allowing them to proceed.
One example of energy coupling using ATP involves a...
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Sugars as Energy Storage Molecules01:10

Sugars as Energy Storage Molecules

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Sugar (a simple carbohydrate) metabolism (chemical reactions) is a classic example of the many cellular processes that use and produce energy. Living things consume sugar as a major energy source because sugar molecules have considerable energy stored within their bonds. Consumed carbohydrates have their origins in photosynthesizing organisms like plants. During photosynthesis, plants use the energy of sunlight to convert carbon dioxide gas into sugar molecules, like glucose. Because this...
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Fats as Energy Storage Molecules01:06

Fats as Energy Storage Molecules

27.0K
Triglycerides are a form of long-term energy storage molecules. They are made of glycerol and three fatty acids. To obtain energy from fat, triglycerides must first be broken down by hydrolysis into their two principal components, fatty acids and glycerol. This process, called lipolysis, takes place in the cytoplasm. The resulting fatty acids are oxidized by β-oxidation into acetyl-CoA, which is used by the Krebs cycle. The glycerol that is released from triglycerides after lipolysis...
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Buffer Effectiveness02:19

Buffer Effectiveness

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Buffer solutions do not have an unlimited capacity to keep the pH relatively constant . Instead, the ability of a buffer solution to resist changes in pH relies on the presence of appreciable amounts of its conjugate weak acid-base pair. When enough strong acid or base is added to substantially lower the concentration of either member of the buffer pair, the buffering action within the solution is compromised.
The buffer capacity is the amount of acid or base that can be added to a given volume...
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Do-It-Yourself Device for Recovery of Cryopreserved Samples Accidentally Dropped into Cryogenic Storage Tanks
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Hydrogel Cryopreservation System: An Effective Method for Cell Storage.

Chaocan Zhang1, Youliang Zhou2, Li Zhang3

  • 1School of Materials Science and Engineering, Wuhan University of Technology, Wuhan 430070, China. polymers@whut.edu.cn.

International Journal of Molecular Sciences
|October 28, 2018
PubMed
Summary
This summary is machine-generated.

Novel hydrogel systems offer improved cell cryopreservation by minimizing damage during thawing. These advanced methods protect cell viability for future transplantation and tissue engineering applications.

Keywords:
cell storagecryopreservationhydrogelsupramolecular gel

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Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Cryobiology

Background:

  • Living cells are crucial for cell transplantation and tissue engineering, requiring methods for long-term storage with high viability.
  • Current cryopreservation techniques face challenges including solution injury, ice injury, recrystallization, osmotic injury during thawing, and cryoprotectant cytotoxicity.
  • Effective cell preservation is essential for advancing regenerative medicine and therapeutic applications.

Purpose of the Study:

  • To review novel gel cryopreservation systems for enhancing cell viability and functionality.
  • To explore the advantages of natural, synthetic, and supramolecular hydrogel systems in cell cryopreservation.
  • To highlight the potential of hydrogel-based cryopreservation for future clinical applications.

Main Methods:

  • Review of natural polymer hydrogel cryopreservation systems.
  • Analysis of synthetic polymer hydrogel cryopreservation systems.
  • Examination of supramolecular hydrogel cryopreservation systems.

Main Results:

  • Hydrogel systems, owing to their 3D network structure, offer improved biocompatibility, designability, and versatility.
  • These hydrogel methods can effectively confine ice crystal growth and reduce osmotic shock during cell encapsulation.
  • Different hydrogel cryopreservation approaches demonstrate potential in mitigating damage associated with traditional methods.

Conclusions:

  • Novel hydrogel cryopreservation systems present a promising strategy to overcome limitations of conventional cell preservation.
  • The unique properties of hydrogels enhance cell protection against cryodamage, preserving cell activity and function.
  • Future clinical research and therapy will likely benefit from the integration of various hydrogel-based cryopreservation methods for complex cells and tissues.