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Updated: Feb 3, 2026
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A Modified Precipitation Method to Isolate Urinary Exosomes
Published on: January 16, 2015
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Efficient method for isolation of exosomes from raw bovine milk
Marika Yamauchi1, Kaori Shimizu1, Matiur Rahman1,2,3
1a Laboratory of Food and Environmental Hygiene, Cooperative Department of Veterinary Medicine , Gifu University , Gifu, Japan.
Drug Development and Industrial Pharmacy
|October 28, 2018
Summary
Isoelectric precipitation (IP) offers a faster method for isolating bovine milk exosomes by removing caseins. This technique yields high-quality exosomes comparable to ultracentrifugation, aiding dairy cattle research and drug delivery systems.
Area of Science:
- Biochemistry
- Nanotechnology
- Veterinary Science
Background:
- Exosomes in bovine milk are valuable for research and therapeutics.
- Current isolation methods like ultracentrifugation are time-consuming and complex.
- Caseins, abundant milk proteins, hinder efficient exosome isolation.
Purpose of the Study:
- To develop a rapid and simple method for isolating exosomes from raw bovine milk.
- To compare the quality of exosomes isolated by isoelectric precipitation (IP) with ultracentrifugation (UC).
Main Methods:
- Isoelectric precipitation (IP) using hydrochloric acid (HCl) to remove caseins.
- Analysis of exosome morphology via electron microscopy.
- Characterization of particle size, surface charge (tunable resistive pulse sensing - TRPS), and surface proteins (Western blot - WB).
Main Results:
- IP effectively removed caseins, reducing isolation time.
- Electron microscopy showed similar morphology between IP and UC isolated exosomes, with minimal breakage.
- TRPS and WB analyses confirmed no significant differences in particle size, surface charge, or key exosome surface proteins (MFG-E8, CD63).
Conclusions:
- Isoelectric precipitation (IP) is a viable, efficient method for isolating bovine milk exosomes.
- This rapid method simplifies exosome isolation, supporting research in dairy cattle health and human drug delivery systems.
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