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Updated: Feb 3, 2026

Human Dupuytren's Ex Vivo Culture for the Study of Myofibroblasts and Extracellular Matrix Interactions
Published on: April 18, 2015
Interaction Between Skeletal Muscle Cells and Extracellular Matrix Proteins Using a Serum Free Culture System
Danielle E Dye1, Beverley F Kinnear1, Vishal Chaturvedi1
1School of Pharmacy and Biomedical Science, Curtin Health Innovation Research Institute, Faculty of Health Science, Curtin University, Bentley, WA, Australia.
Abstract:
The ability to grow C2C12 myoblasts in a completely defined, serum free medium enables researchers to investigate the role of specific factors in myoblast proliferation, migration, fusion, and differentiation without the confounding effects of serum. The use of defined, animal free in vitro culture systems will improve reproducibility between research groups and may also enhance translation of tissue engineering techniques into clinical applications. Here, we describe the use and characterization of a serum free culture system for C2C12 myoblasts using standard tissue culture medium and readily available, defined growth factors and supplements.
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