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Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin
Published on: August 14, 2018
First report of molecular characterization and sequence phylogenetic analysis of Linguatula serrata from India
Vikrant Sudan1, Daya Shanker1, Amit Kumar Jaiswal1
1Department of Veterinary Parasitology, College of Veterinary Sciences and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa VigyanVishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura-281001, India.
Abstract:
Linguatula serrata is a pentastomid which is worldwide in distribution. However, a very few references are reported from India. In the present study, the cox I gene of L. serrata nymphs, originally isolated from mesenteric lymph nodes of buffaloes, was amplified and custom sequenced. Based on sequence analysis, two haplotypes were noticed and were subsequently submitted in NCBI database. The sequences were also compared with the other sequences available in the pubmed and phylogenetic analysis coupled with nucleotide homologies were commutated. The studied Indian isolates were found closer to Bangladesh and Iran isolates. This is the first report of molecular characterization of L. serrata from India.
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