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Related Experiment Videos

Structural changes of 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH) treated shrimp tropomyosin decrease

Liangtao Lv1, Hong Lin1, Zhenxing Li1

  • 1Food Safety Laboratory, College of Food Science and Engineering, Ocean University of China, Qingdao 266003, PR China.

Food Chemistry
|October 31, 2018
PubMed
Summary

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This summary is machine-generated.

Adding 2,2-azobis(2-amidinopropane) dihydrochloride (AAPH) to shrimp tropomyosin (TM) induces aggregation, altering its structure and reducing its allergenicity. This structural change diminishes immune responses, offering insights into food allergy management.

Area of Science:

  • Food science
  • Allergen analysis
  • Protein chemistry

Background:

  • Shrimp tropomyosin (TM) is a major seafood allergen.
  • Understanding structural changes in TM is crucial for mitigating allergic reactions.

Purpose of the Study:

  • To investigate the impact of 2,2-azobis(2-amidinopropane) dihydrochloride (AAPH) on shrimp TM structure and allergenicity.
  • To evaluate AAPH-induced modifications in TM's conformational and immunological properties.

Main Methods:

  • Structural analysis using SDS-PAGE, fluorescence, circular dichroism (CD), and ultraviolet (UV) spectroscopy.
  • Allergenicity assessment via in vivo (mouse model) and in vitro (immunoblot, competitive inhibition ELISA, RBL-2H3 cell assay) methods.

Main Results:

Keywords:
1-Anilino-8-naphthalene-sulfonate (PubChem CID: 1369)3,3′,5,5′-Tetramethylbenzidine (PubChem CID: 41206)AAPHAcrylamide (PubChem CID: 6579)AllergenicityBrilliant Blue R (PubChem CID: 61365)DL-Dithiothreitol (PubChem CID: 446094)DL-lysine (PubChem CID: 866)L-leucine (PubChem CID: 6106)Protein oxidationShrimp tropomyosin

Related Experiment Videos

  • AAPH induced significant aggregation of shrimp TM, leading to conformational changes.
  • Aggregated TM exhibited reduced IgG/IgE binding capacity, with IC50 values increasing from 0.086 to 46.2 μg/ml.
  • AAPH treatment decreased the release of β-hexosaminidase and histamine from RBL-2H3 cells.
  • In a mouse model, AAPH-treated TM reduced IgE/IgG1 levels, histamine, and mMCP-1 release.

Conclusions:

  • AAPH-induced aggregation of shrimp TM causes structural alterations.
  • These structural changes are directly correlated with a significant decrease in TM's allergenicity.