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Updated: Feb 3, 2026

A Loop-mediated Isothermal Amplification LAMP Assay for Rapid Identification of Bemisia tabaci
Published on: October 29, 2018
A rapid sample-to-answer analytical detection of genetically modified papaya using loop-mediated isothermal
Jacky Loo1, Grace Wing-Chiu But2, Ho-Chin Kwok3
1School of Life Sciences, The Chinese University of Hong Kong, Hong Kong; Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong.
Abstract:
With genetically modified (GM) food circulating on the market, a rapid transgenic food screening method is needed to protect consumer rights. The on-site screening efficiency of GM food testing is low. We report rapid sample-to-answer detection of GM papayas with loop-mediated isothermal amplification (LAMP) and a compact, portable, integrated microfluidic platform using microfluidic lab-on-a-disc (LOAD). GM samples were differentiated from non-GM papaya, based on the detection of a specific GM (P-35S (Cauliflower mosaic virus 35S promoter)) and non-GM DNA marker (papain) in 15 min. The detection limits for DNA and juice from papaya were 10 pg/µL and 0.02 µL, respectively. Our LOAD platform is a simple and robust solution for GM screening, which is anticipated to be a foundation for on-site testing of transgenic food.
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