Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Experimental Determination of Chemical Formula02:37

Experimental Determination of Chemical Formula

47.1K
The elemental makeup of a compound defines its chemical identity, and chemical formulas are the most concise way of representing this elemental makeup. When a compound’s formula is unknown, measuring the mass of its constituent elements is often the first step in determining the formula experimentally.
47.1K
Correlation of Experimental Data01:23

Correlation of Experimental Data

488
Dimensional analysis simplifies complex physical problems and guides experimental investigations, but it does not provide complete solutions. It identifies the dimensionless groups that influence a phenomenon, but experimental data is needed to establish the specific relationships and validate theoretical predictions.
For example, a spherical particle moving through a viscous fluid experiences drag. Dimensional analysis shows that the drag force depends on the particle's diameter, velocity,...
488
X-ray Crystallography02:18

X-ray Crystallography

26.2K
The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
26.2K
One-Compartment Open Model: Urinary Excretion Data and Determination of k01:11

One-Compartment Open Model: Urinary Excretion Data and Determination of k

629
The one-compartment open model leverages urinary excretion data to estimate renal clearance, which gauges the kidney's capacity to expel a drug. This method offers several benefits, including directly measuring drug elimination and assessing the kidney's contribution to overall drug clearance. However, this approach has limitations. It assumes sole renal excretion of the drug, which is not true for all drugs. Accurate urinary excretion and plasma drug concentration measurement can also...
629
Scatter Plot01:15

Scatter Plot

11.4K
The most common and easiest way to display the relationship between two variables, x and y, is a scatter plot. A scatter plot shows the direction of a relationship between the variables. A clear direction happens when there is either:
11.4K
X-ray Imaging01:24

X-ray Imaging

10.2K
German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with...
10.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dichography: two-frame ultrafast imaging from a single diffraction pattern.

Nature communications·2026
Same author

Molecular magnetic X-ray scattering with ultrashort X-ray free-electron lasers: from spin-orbit dynamics to Berry phase detection.

Physical chemistry chemical physics : PCCP·2026
Same author

Liquid jet capabilities for ultrafast chemistry at the SwissFEL Alvra instrument.

Journal of synchrotron radiation·2026
Same author

Experimental Data Driven AI Framework for Flexible Protein Conformational Reconstruction.

bioRxiv : the preprint server for biology·2026
Same author

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Acta crystallographica. Section D, Structural biology·2026
Same author

Assessing the problem of excitation light scattering in high-viscosity jets used in serial crystallography sample delivery.

Journal of applied crystallography·2026
Same journal

Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Tomographic imaging of superconducting order using particle-hole interference.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Analytical modeling for suction cup designs for skin-interfaced wearable devices.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Improving cell-free metabolism through direct integration of artificial respiratory chains.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

Related Experiment Video

Updated: Feb 3, 2026

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling
10:27

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling

Published on: October 21, 2018

13.0K

Ab initio structure determination from experimental fluctuation X-ray scattering data.

Kanupriya Pande1,2, Jeffrey J Donatelli1,3, Erik Malmerberg1,2,4

  • 1Center for Advanced Mathematics for Energy Research Applications, Lawrence Berkeley National Laboratory, Berkeley, CA 94720-8142.

Proceedings of the National Academy of Sciences of the United States of America
|October 31, 2018
PubMed
Summary
This summary is machine-generated.

Free-electron laser (FEL) fluctuation X-ray scattering (FXS) advances biological particle analysis. This study demonstrates FEL-FXS feasibility and a noise-filtering technique for accurate electron density reconstruction of viruses.

Keywords:
fluctuation X-ray scatteringfree-electron lasersmall-angle scattering

More Related Videos

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
07:19

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

13.4K
Analysis of SEC-SAXS data via EFA deconvolution and Scatter
10:59

Analysis of SEC-SAXS data via EFA deconvolution and Scatter

Published on: January 28, 2021

9.9K

Related Experiment Videos

Last Updated: Feb 3, 2026

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling
10:27

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling

Published on: October 21, 2018

13.0K
Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
07:19

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

13.4K
Analysis of SEC-SAXS data via EFA deconvolution and Scatter
10:59

Analysis of SEC-SAXS data via EFA deconvolution and Scatter

Published on: January 28, 2021

9.9K

Area of Science:

  • Biophysics
  • Structural Biology
  • X-ray scattering

Background:

  • Traditional solution scattering methods yield limited data.
  • Free-electron lasers (FELs) offer ultrashort X-ray pulses for advanced scattering experiments.
  • Fluctuation X-ray scattering (FXS) utilizes these pulses to capture more detailed particle information.

Purpose of the Study:

  • To demonstrate the practical application of FEL-based FXS on a biological system.
  • To develop and describe data-processing techniques for robust FXS data acquisition.
  • To showcase ab initio electron density reconstruction using FEL-FXS data.

Main Methods:

  • Utilizing ultrashort X-ray exposures from a free-electron laser (FEL).
  • Collecting X-ray solution scattering data from particles in solution.
  • Implementing an iterative noise-filtering technique to reduce data requirements.
  • Performing ab initio electron density reconstruction.

Main Results:

  • Demonstrated the feasibility of FEL-based FXS for biological multiple-particle systems.
  • Developed data-processing techniques to extract robust FXS data.
  • Successfully reduced the number of required snapshots through noise filtering.
  • Achieved ab initio electron density reconstruction of Paramecium bursaria Chlorella virus (PBCV-1).

Conclusions:

  • FEL-based FXS is a viable technique for studying biological particles.
  • Advanced data processing, including noise filtering, enhances FXS data quality and efficiency.
  • This method enables detailed structural insights, such as virus electron density reconstruction.