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Updated: Feb 3, 2026

Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron
Published on: September 23, 2020
The hit-and-return system enables efficient time-resolved serial synchrotron crystallography
Eike C Schulz1, Pedram Mehrabi1, Henrike M Müller-Werkmeister1,2
1Department for Atomically Resolved Dynamics, Max Planck Institute for Structure and Dynamics of Matter, Hamburg, Germany.
Abstract:
We present a 'hit-and-return' (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals.
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