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Detecting Behavioral Deficits in Rats After Traumatic Brain Injury
Published on: January 30, 2018
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Detection of a Traumatic Brain Injury Biomarker at the 10 fg/mL Level
Anup S Mathew1, Xuyang Shi1, Siu-Tung Yau2,3
1Department of Electrical Engineering and Computer Science, Cleveland State University, Cleveland, OH, 44115, USA.
Molecular Diagnosis & Therapy
|November 1, 2018
Summary
An ultrasensitive electrochemical immunoassay detects S100B, a key biomarker for mild traumatic brain injury (mTBI). This method offers early diagnosis potential for mTBI by detecting minute protein amounts in body fluids.
Area of Science:
- Biomarker Detection
- Electrochemical Immunoassay
- Neuroscience Diagnostics
Background:
- Early diagnosis and prognosis of mild traumatic brain injury (mTBI) rely on detecting minute protein biomarkers in body fluids.
- S100B is a well-studied potential biomarker for mTBI diagnosis.
- Ultrasensitive detection methods are crucial for identifying these low-concentration biomarkers.
Purpose of the Study:
- To develop and validate an ultrasensitive detection method for S100B, a biomarker for mTBI.
- To assess the feasibility of using this method for early mTBI diagnosis.
- To establish a platform for detecting various mTBI markers.
Main Methods:
- A modified electrochemical immunoassay technique utilizing voltage-controlled intrinsic current signal amplification.
- Formation of a sandwich immune complex of S100B on a screen-printed electrode's working electrode.
- Gating voltage application for amplifying the current signal through the immune complex.
Main Results:
- Detection of S100B in human serum with a limit of detection as low as 10 fg/mL.
- Calibration curves demonstrated a wide dynamic range, spanning four orders of magnitude (10 fg/mL to 10 ng/mL).
- Specificity was confirmed by differentiating S100B from TAU protein, another mTBI marker.
Conclusions:
- The field effect enzymatic detection (FEED)-based immunoassay is feasible for detecting extremely low concentrations of mTBI markers in human serum.
- This ultrasensitive method shows promise for the early diagnosis and prognosis of mTBI.
- The developed method can serve as a versatile platform for a broader range of mTBI-related biomarkers.
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