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Evaluating Stress Granules in Pancreatic Cancer In Vitro and In Vivo
Edward Sim1, Elena Irollo1, Elda Grabocka2
1Department of Cancer Biology, Thomas Jefferson University, Philadelphia, PA, USA.
Abstract:
Stress granules are nonmembranous organelles that function as a stress-adaptation mechanism. We have recently shown that stress granules are mobilized by mutant KRAS pancreatic cancer cells under stress to enhance tumor fitness and survival. In this chapter, we outline a method for inducing, detecting, and quantifying stress granules in pancreatic cancer cells in vitro and in vivo. This method can be utilized to better understand the mechanisms driving stress granule formation and their role in pancreatic tumorigenesis.
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