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The biological functions of target genes in pan-cancers and cell lines were predicted by miR-375 microarray data from

Jiang-Hui Zeng1, Xu-Zhi Liang2, Hui-Hua Lan3

  • 1Department of ClinicaHl Laboratory, The Third Affiliated Hospital of Guangxi Medical University/Nanning Second People's Hospital, Nanning, Guangxi Zhuang Autonomous Region, P. R. China.

Plos One
|November 1, 2018
PubMed
Abstract

Insights

This study reveals microRNA-375 (miR-375) targets key genes involved in amino acid metabolism, offering insights into cancer mechanisms. miR-375 negatively correlates with MAT2B, a potential therapeutic target in various cancers.

Area of Science:

  • Oncology
  • Molecular Biology
  • Bioinformatics

Background:

  • MicroRNAs (miRNAs) are small non-coding RNAs regulating gene expression.
  • Evidence links miRNA dysregulation to oncogenesis and cancer pathogenesis.
  • MicroRNA-375 (miR-375) is implicated in various cancers, necessitating mechanistic exploration.

Purpose of the Study:

  • To investigate the molecular mechanisms of miR-375 in diverse human cancers.
  • To identify common target genes of miR-375 across different tumor types.
  • To elucidate the functional role of miR-375 in cancer development.

Main Methods:

  • Microarray data analysis from Gene Expression Omnibus (GEO) for miR-375 expression.
  • Target gene prediction using miRWalk2.0 and Robust Rank Aggregation (RRA).
  • In silico analyses including PPI, GO, and KEGG pathway enrichment.
  • Validation using The Cancer Genome Atlas (TCGA) data.

Main Results:

  • Identified five key miR-375 target genes: VASN, MAT2B, HERPUD1, TPAPPC6B, and TAT.
  • Enrichment analysis revealed target genes involved in amino acid biosynthesis and metabolism.
  • Demonstrated a negative correlation between miR-375 and MAT2B in five TCGA-validated cancers.

Conclusions:

  • This study provides a novel perspective on miR-375's molecular mechanisms in human tumors through common target gene identification.
  • MAT2B emerges as a significant miR-375 target potentially involved in cancer progression.
  • Findings highlight miR-375's role in amino acid metabolism and its implications for cancer therapy.

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