Related Experiment Video
Updated: Feb 3, 2026

A Quantitative Glycomics and Proteomics Combined Purification Strategy
Published on: March 8, 2016
Anthranilic Acid as a Versatile Fluorescent Tag and Linker for Functional Glycomics
Yuyang Zhu1, Xueyun Liu1, Ying Zhang1,2
1Department of Biochemistry, Emory Comprehensive Glycomics Core , Emory University School of Medicine , Atlanta , Georgia 30322 , United States.
Abstract:
The advancement of glycoscience is critically dependent on the access to a large number of glycans for their functional study. Naturally occurring glycans are considered a viable source for diverse and biologically relevant glycan libraries. A mixture of free reducing glycans released from natural sources can be fluorescently tagged and separated by chromatography to produce a natural glycan library. Anthranilic acid (AA) has been widely used to fluorescently tag reducing glycans for HPLC or LC/MS analysis. However, AA conjugated glycans are not efficiently immobilized on microarray slides due to the lack of a primary alkylamine functional group. In this study, we have developed simple and efficient chemistry for bioconjugation and further functionalization of glycan-AA conjugates. This new approach enables quick preparation of glycan microarrays and neoglycoproteins from glycan-AA conjugates, which can be separated by weak anion exchange (WAX) and C18 reversed-phase HPLC.
Related Concept Videos
Cytoskeletal Linker Proteins - Plakins
Composition of Polyprotic Acid Solutions as a Function of pH
A graph with the alpha values is plotted against the volume of...
Amino acids
Functional Groups
Nucleic Acids
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
Nucleic acids
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...

