Related Experiment Video
Updated: Feb 14, 2026

Detection of Viruses from Bioaerosols Using Anion Exchange Resin
Published on: August 22, 2018
Comparative Study of Cation-Exchange Resins for High-Efficiency Preparation of Bovine Lactoferrin and its N-glycomic
Xiaoqin Wang1, Yujie Liao1, Xiaoxiao Guo1
1Shaanxi Natural Carbohydrate Resource Engineering Research Center, Key Laboratory of Glycobiology and Glycoengineering of Xi'an, College of Food Science and Technology, Northwest University, Xi'an 710069, China.
Abstract:
Bovine lactoferrin (bLf) is an essential additive in infant formula, with its bioactivity closely related to N-glycosylation. Currently, the production cost of lactoferrin remains high. This study compared eight ion-exchange resins and identified DoWex and 732 as optimal for bLf purification. Yields reached 4.46% for DoWex and 5.25% for 732, with purities exceeding 85% for both resins. Isomer-specific N-glycan analysis using HILIC-MS/MS revealed 58 distinct N-glycans in mature bLf, including 28 neutral and 30 acidic glycans, with the acidic N-glycans predominantly modified by α2,6-linked Neu5Ac. The bLf prepared by 732 resin shared >97% of the neutral and >93% of the acidic glycans with that of DoWex. Notably, the price of 732 resin is only one-18th that of DoWex, representing a significant reduction in production costs and making it promising in future large-scale manufacturing. This work facilitates efficient lactoferrin production and provides further studies on the relationships between glycans and their functions.
Related Concept Videos
Comparing the Survival Analysis of Two or More Groups
Social Exchange Theory
Social Exchange Theory
Gas Exchange and Transport
Capillary Exchange
Ion Exchange

