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Related Experiment Video

Updated: Feb 3, 2026

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis
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Reference Values for WBC Differential by Hematoflow Analysis.

Marion Magierowicz1, Nicolas Lechevalier2, Nicolas Freynet3

  • 1Laboratoire Hématologie, Centre Hospitalier Universitaire Rennes, Rennes, France.

American Journal of Clinical Pathology
|November 2, 2018
PubMed
Summary

This study defines reference values for white blood cell (WBC) differentials using flow cytometry, crucial for automated validation in clinical laboratories. These normal values aid in flagging abnormal results for patient care.

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Area of Science:

  • Clinical Laboratory Science
  • Immunology
  • Hematology

Background:

  • Flow cytometry with monoclonal antibodies is increasingly used for white blood cell (WBC) differentials in routine laboratory workflows.
  • Establishing reference values is essential for automated result validation and accurate clinical interpretation.

Purpose of the Study:

  • To define reference values for WBC differentials analyzed by flow cytometry.
  • To support the implementation of automated validation processes in clinical laboratories.

Main Methods:

  • Analysis of 584 patient samples from three hospitals.
  • Utilized the Hematoflow solution for flow cytometry-based WBC differential analysis.

Main Results:

  • Reference values were established for five distinct age groups: 0-5, 6-11, 12-19, 20-69, and over 69 years.
  • The data provides age-specific normal ranges for WBC populations.

Conclusions:

  • The defined reference values are critical for setting thresholds for automatic sample validation.
  • These normal values will facilitate the accurate flagging of pathological samples analyzed by flow cytometry.