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Updated: Feb 3, 2026

Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages
Published on: April 20, 2018
Tissue Unit-ed: Lung Cells Team up to Drive Alveolar Macrophage Development
Charlotte L Scott1, Martin Guilliams2
1Laboratory of Myeloid Cell Ontogeny and Functional Specialization, VIB-UGent Centre for Inflammation Research, Ghent, Belgium; Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium; Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, UK.
Abstract:
Using single-cell RNA sequencing of both immune and non-immune cells in the developing lung, Cohen et al. map candidate cell-cell interactions during alveolar macrophage development. This revealed potential cross-talk between epithelial cells, ILC2s, basophils, and the developing macrophages, which were validated both in vitro and in vivo.
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