Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

B and T lymphocytes: methodology and normal ranges.

A B Glassman, C E Bennett

    Annals of Clinical and Laboratory Science
    |November 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Fasciola gigantica: Ultrastructure and cytochemistry of tegumental secretory activity and extracellular vesicle formation in juvenile flukes migrating in mouse liver.

    Veterinary parasitology·2026
    Same author

    Moisture, anisotropy, stress state, and strain rate effects on bighorn sheep horn keratin mechanical properties.

    Acta biomaterialia·2016
    Same author

    Epidemiology of scabies prevalence in the U.K. from general practice records.

    The British journal of dermatology·2011
    Same author

    Metformin reduces C-reactive protein but not complement factor C3 in overweight patients with Type 2 diabetes mellitus.

    Diabetic medicine : a journal of the British Diabetic Association·2005
    Same author

    Reticulum cell sarcoma of lymph node with mixed dendritic and fibroblastic features.

    Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc·2001
    Same author

    Studies on the synthesis of landomycin A: synthesis and glycosidation reactions of L-rhodinosyl acetate derivatives.

    The Journal of organic chemistry·2001

    This study details methods for differentiating B and T lymphocytes, crucial for diagnosing diseases. Preliminary findings show significant differences in T cell populations in diabetics compared to healthy individuals.

    Area of Science:

    • Immunology
    • Clinical Laboratory Science

    Background:

    • Lymphocyte subpopulations, including B and T cells, are vital for immune function.
    • Differential counts aid in diagnosing immunodeficiency, lymphoproliferative disorders, and other conditions.
    • Clinical demand has led to the routine use of these assays in general laboratories.

    Purpose of the Study:

    • To describe specific methodologies for differentiating B and T lymphocyte subpopulations.
    • To establish reference ranges for T and B cell populations in healthy individuals.
    • To investigate potential alterations in lymphocyte populations in specific disease states.

    Main Methods:

    • Utilized cell markers for the separation and differentiation of B and T lymphocytes.
    • Performed differential lymphocyte subpopulation counts on a cohort of 30 ambulatory normal subjects.

    Related Experiment Videos

  • Examined lymphocyte cell line distributions in a family with a high incidence of carcinoma.
  • Analyzed T cell populations in patients from a diabetic clinic.

    • Main Results:

    • Established mean T cell population at 68.1% ± 4.2% and mean B cell population at 8.4% ± 2.1% in normal subjects.
    • Observed a bi-modal distribution of T and B lymphocyte cell lines in a family with high carcinoma incidence.
    • Identified a statistically significant difference in T cell populations between diabetic patients and normal controls.

    Conclusions:

    • The described methodology enables reliable differentiation of B and T lymphocyte subpopulations.
    • Lymphocyte subpopulation analysis may serve as a valuable diagnostic tool for various diseases.
    • Preliminary data suggests a potential link between altered T cell populations and diabetes mellitus.