Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

9.6K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
9.6K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

26.4K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
26.4K
Translational Regulation01:29

Translational Regulation

614
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
614
Negative Regulator Molecules01:23

Negative Regulator Molecules

38.5K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.5K
Regulated Protein Degradation02:58

Regulated Protein Degradation

8.9K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
8.9K
Translation01:31

Translation

156.4K
Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of...
156.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Decoding The Epitranscriptome: In Silico Insights Into m6A Regulatory Network In Breast Cancer.

Journal of visualized experiments : JoVE·2026
Same author

Efficacy of Vitamin D Supplementation to Alleviate Premenstrual Syndrome Symptoms: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.

Journal of clinical medicine·2026
Same author

Correcting photoreceptor diseases at their source: CRISPR strategies for cone-rod dystrophy and achromatopsia.

Experimental eye research·2026
Same author

Association of inflammation with sex hormones and vitamin D in women: Findings from NHANES (2021-2023).

Scottish medical journal·2026
Same author

Decitabine Induces Subtype-Specific Epigenomic Remodeling and Perturbs Age-Associated Regulatory CpGs in Breast Cancer.

Cancer informatics·2026
Same author

A Vascular-Extracellular Matrix Molecular Program Identifies High-Risk Diffuse Glioma Across Independent Multi-Omics.

Cancers·2026

Related Experiment Video

Updated: Feb 3, 2026

Purification of Ubiquitinated p53 Proteins from Mammalian Cells
10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

2.8K

KDM5A Regulates a Translational Program that Controls p53 Protein Expression.

Dongli Hu1, Carolyn Jablonowski1, Pei-Hsin Cheng1

  • 1Department of Surgery, St Jude Children's Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105, USA.

Iscience
|November 3, 2018
PubMed
Summary
This summary is machine-generated.

Histone demethylase KDM5A negatively regulates the p53 tumor suppressor pathway by inhibiting p53 translation. Its amplification in cancers is mutually exclusive to p53 mutations, suggesting KDM5A as a potential therapeutic target.

Keywords:
CancerMolecular Mechanism of Gene Regulation

More Related Videos

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation
08:00

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

1.1K
Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
12:26

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Published on: February 12, 2022

5.9K

Related Experiment Videos

Last Updated: Feb 3, 2026

Purification of Ubiquitinated p53 Proteins from Mammalian Cells
10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

2.8K
Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation
08:00

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

1.1K
Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
12:26

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Published on: February 12, 2022

5.9K

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • The p53 tumor suppressor pathway is crucial for preventing cancer but is inactivated in many human cancers.
  • Some cancer types lack common p53 signaling alterations, indicating alternative regulatory mechanisms.
  • Histone demethylase KDM5A emerges as a key player in regulating p53 activity.

Purpose of the Study:

  • To investigate the role of histone demethylase KDM5A in p53 pathway regulation.
  • To explore the relationship between KDM5A amplification, p53 mutation status, and cancer progression.
  • To elucidate the mechanism by which KDM5A affects p53 activity and cancer growth.

Main Methods:

  • Analysis of KDM5A amplification in various cancer types.
  • Assessment of KDM5A's impact on p53 translation and activity.
  • Genetic deletion of KDM5A in cancer cell lines.
  • Investigation of the regulatory loop involving p53, miR-34, and KDM5A.

Main Results:

  • KDM5A is significantly amplified in multiple cancers, often mutually exclusive to p53 mutations.
  • KDM5A inhibits p53 translation by suppressing eukaryotic translation initiation genes.
  • KDM5A deletion upregulates p53 and suppresses tumor growth in a p53-dependent manner.
  • A regulatory loop exists where miR-34 induction suppresses KDM5A.

Conclusions:

  • KDM5A acts as a negative regulator of p53 by inhibiting its translation.
  • KDM5A amplification represents an alternative mechanism of p53 pathway inactivation in cancer.
  • The p53-miR-34-KDM5A axis offers novel insights into cancer progression and potential therapeutic strategies.