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Updated: Feb 3, 2026

Intravital Longitudinal Imaging of Vascular Dynamics in the Calvarial Bone Marrow
Published on: April 11, 2025
High-speed polarization imaging of dynamic collagen fiber realignment in tendon-to-bone insertion region
Xianyu Wu1, Mark Pankow1, Hsiao-Ying Shadow Huang1
1North Carolina State University, Department of Mechanical and Aerospace Engineering, Raleigh, North, United States.
Abstract:
A high-speed polarization imaging instrument is demonstrated to be capable of measuring the collagen fiber alignment orientation and alignment strength during high-displacement rate dynamic loading at acquisition rates up to 10 kHz. The implementation of a high-speed rotating quarter wave plate and high-speed camera in the imaging system allows a minimum measurement acquisition time of 6 ms. Sliced tendon-to-bone insertion samples are loaded using a modified drop tower with an average maximum displacement rate of 1.25 m / s, and imaged using a high-speed polarization imaging instrument. The generated collagen fiber alignment angle and strength maps indicate the localized deformation and fiber realignment in tendon-to-bone samples during dynamic loading. The results demonstrate a viable experimental method to monitor collagen fiber realignment in biological tissue under high-displacement rate dynamic loading.
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