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Multiplex immunoassays using surface modification-mediated porous layer open tubular capillary.
Xingda Song1, Rongbin Nie1, Xiaoxia Liu2
1Key Laboratory of Nanobiosensing and Nanobioanalysis at Universities of Jilin Province, Department of Chemistry, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province, 130024, China.
Analytica Chimica Acta
|November 6, 2018
Summary
We developed a novel porous layer open tubular (PLOT) capillary for enhanced immunoassays. This sensitive, rapid, and multiplex immunosensor significantly improves cancer biomarker detection in clinical samples.
Area of Science:
- Analytical Chemistry
- Biomedical Engineering
- Materials Science
Background:
- Immunoassays are crucial for detecting biomarkers but often suffer from low sensitivity and long analysis times.
- Conventional capillary sensors have limited surface area for antibody immobilization, hindering assay performance.
- There is a need for improved immunoassay platforms that offer enhanced sensitivity, speed, and multiplexing capabilities.
Purpose of the Study:
- To develop an innovative surface modification-mediated porous layer open tubular (PLOT) capillary for enhanced immunoassay performance.
- To create a sensitive, rapid, and multiplexed immunosensor for simultaneous detection of cancer biomarkers.
- To evaluate the accuracy and efficiency of the PLOT capillary-based immunosensor in clinical serum samples.
Main Methods:
- Fabrication of PLOT capillaries using an in situ biphasic reaction for a 3D porous structure.
- Immobilization of antibodies onto the PLOT capillary surface to increase surface-area-to-volume ratio.
- Development of a multiplexed immunosensor by connecting PLOT capillaries for simultaneous detection of alpha fetoprotein (AFP) and carcinoembryonic antigen (CEA).
Main Results:
- The PLOT capillary exhibited a homogeneous and porous structure, ideal for antibody immobilization.
- The PLOT immunosensor demonstrated a 10-fold improvement in detection sensitivity compared to bare-capillary sensors.
- Simultaneous detection of AFP and CEA in clinical serum samples was achieved with good accuracy within 1 hour.
Conclusions:
- Surface modification strategies, like the PLOT capillary, are effective in enhancing immunoassay analytical performance.
- The developed PLOT capillary-mediated immunosensor offers a feasible, sensitive, and multi-target assay for cancer biomarker detection.
- This technology holds significant potential for valuable applications in clinical diagnosis and disease monitoring.
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