Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA-only Transposons02:57

DNA-only Transposons

17.5K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
17.5K
Transposons01:24

Transposons

1.9K
Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
1.9K
Adult Stem Cells01:33

Adult Stem Cells

33.9K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
33.9K
Embryonic Stem Cells00:58

Embryonic Stem Cells

32.4K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
32.4K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

28.0K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
28.0K
Healthcare Associated Infections I: Iatrogenic, Exogenic and Endogenic01:26

Healthcare Associated Infections I: Iatrogenic, Exogenic and Endogenic

5.5K
Healthcare-associated infections (HAIs) occur in a healthcare facility while a person receives care for another ailment. This category also includes work-related infections among healthcare staff.
HAIs significantly increase the cost of health care. Extended stays in healthcare institutions, increased disability, increased costs of medications, including specialized antibiotics, and prolonged recovery times add to the patient's expenses and the healthcare institution and funding bodies.
5.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The cytoskeleton regulates cytoophidium dynamics in Drosophila ovaries.

Cell & bioscience·2026
Same author

A Superoxide Anion-Responsive NIR Fluorogenic Probe Unravels Aucubin's Dual-Axis Therapeutic Mechanism in Hypoxic Pulmonary Hypertension via Ferroptosis Suppression and Oxidative Injury Mitigation.

Analytical chemistry·2026
Same author

Molecularly Engineered Dual-Lock Activatable NIR-II Fluorogenic Probe for Precision Tumor Resection and Real-Time Chemotherapy Response Monitoring.

Analytical chemistry·2025
Same author

Annexin A7 enhances TIA1 axonal trafficking to counteract pathological aggregation in neurons.

The EMBO journal·2025
Same author

A protocol for real-time tracking of transposon products and host proteins in Drosophila ovaries.

STAR protocols·2025
Same author

Translesion DNA polymerases Rev1 and PolH promote LTR-retrotransposon transcription by safeguarding Pol II occupancy in both germline and somatic tissues of Drosophila.

Nucleic acids research·2025
Same journal

ARID1A terminates gastric regeneration to prevent cancer.

Developmental cell·2026
Same journal

Myc sustains sex-biased organ zonation in the Drosophila intestine.

Developmental cell·2026
Same journal

Two parallel neuronal circuits involving electrical synapse and DAF-7/TGF-β signaling regulate muscle autophagy in C. elegans.

Developmental cell·2026
Same journal

Menstruation: Once unspoken but now uncovered, one cell type at a time.

Developmental cell·2026
Same journal

The ALS- and FTD-associated proteins annexin A11 and CHMP2B act sequentially in plasma membrane repair.

Developmental cell·2026
Same journal

Organoid modeling of lung branching morphogenesis and epithelial lineage specification.

Developmental cell·2026
See all related articles

Related Experiment Video

Updated: Feb 3, 2026

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
08:24

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

Published on: February 28, 2017

7.5K

A Robust Transposon-Endogenizing Response from Germline Stem Cells.

Sungjin Moon1, Madeline Cassani1, Yu An Lin1

  • 1Department of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, USA.

Developmental Cell
|November 6, 2018
PubMed
Summary
This summary is machine-generated.

Drosophila ovarian stem cells mount a rapid adaptive defense against P element transposon invasions by activating DNA damage checkpoints and piRNA production, ensuring survival and oogenesis resumption.

More Related Videos

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
14:48

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

Published on: August 25, 2018

28.1K
Extended Live Imaging of Female Drosophila melanogaster Germline Stem Cell Niches
07:10

Extended Live Imaging of Female Drosophila melanogaster Germline Stem Cell Niches

Published on: December 20, 2024

1.1K

Related Experiment Videos

Last Updated: Feb 3, 2026

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
08:24

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

Published on: February 28, 2017

7.5K
Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
14:48

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

Published on: August 25, 2018

28.1K
Extended Live Imaging of Female Drosophila melanogaster Germline Stem Cell Niches
07:10

Extended Live Imaging of Female Drosophila melanogaster Germline Stem Cell Niches

Published on: December 20, 2024

1.1K

Area of Science:

  • Genetics
  • Molecular Biology
  • Evolutionary Biology

Background:

  • Transposons, or jumping genes, frequently invade genomes, necessitating host survival mechanisms.
  • The specific host cell types and adaptive strategies employed during initial transposon invasions are not well understood.

Purpose of the Study:

  • To investigate the adaptive responses of host cells to P element transposon invasions.
  • To identify the mechanisms by which germline stem cells survive and counteract transposon activity.

Main Methods:

  • Utilizing Drosophila melanogaster as a model organism.
  • Manipulating P element activity using temperature control.
  • Analyzing DNA damage checkpoints, piRNA production, and oogenesis status.

Main Results:

  • Germline stem cells activate DNA damage checkpoints and piRNA production to endogenize P elements.
  • Temperature effectively modulates P element activity, enabling hyper-activation for study.
  • Drosophila initially halts oogenesis and induces apoptosis but ovarian stem cells activate adaptive responses.
  • Within four days, hosts amplify silencing piRNAs, repair DNA damage, suppress transposons, and resume oogenesis.

Conclusions:

  • Germline stem cells possess a robust adaptive response to P element invasion.
  • This adaptive mechanism, involving DNA damage response and piRNA amplification, allows survival against recurrent transposon invasions.
  • The findings suggest a conserved evolutionary strategy for genome defense.