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Updated: Feb 3, 2026

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR
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Published on: July 30, 2011

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Microarray analysis of altered long non-coding RNA expression profile in liver cancer cells treated by ginsenoside

Wei-Wen Chen1, Ying-Feng Huang1, Zhi-Bing Hu1

  • 1a Medical Laboratories, Guangzhou Twelfth People's Hospital , Guangzhou 510620 , China.

Journal of Asian Natural Products Research
|November 6, 2018
PubMed

Insights

Ginsenoside Rh2 significantly altered long noncoding RNA (lncRNA) expression in liver cancer cells. This study identifies potential new targets for hepatocellular carcinoma (HCC) therapy.

Area of Science:

  • Molecular Biology
  • Genomics
  • Cancer Research

Background:

  • Hepatocellular carcinoma (HCC) is a major global health concern.
  • Ginsenoside Rh2, a natural compound, shows potential anti-cancer properties.
  • Understanding lncRNA roles in cancer is crucial for developing new therapies.

Purpose of the Study:

  • To investigate the effect of ginsenoside Rh2 on lncRNA and mRNA expression in HepG2 cells.
  • To identify differentially expressed lncRNAs in response to ginsenoside Rh2 treatment.
  • To explore potential therapeutic targets for HCC.

Main Methods:

  • Microarray analysis was used to profile lncRNA and mRNA expression in HepG2 cells.
  • Cells were treated with 20 μg/ml ginsenoside Rh2 or left untreated.
  • Quantitative real-time polymerase chain reaction (qRT-PCR) was used for validation.

Main Results:

  • Ginsenoside Rh2 treatment led to significant changes in lncRNA expression.
  • 618 lncRNAs were upregulated and 161 were downregulated in treated HepG2 cells.
  • Three differentially expressed lncRNAs were successfully validated using qRT-PCR.

Conclusions:

  • Ginsenoside Rh2 modulates lncRNA expression in hepatocellular carcinoma cells.
  • The identified differentially expressed lncRNAs may serve as novel therapeutic targets for HCC.
  • Further research into ginsenoside Rh2 could lead to effective anti-cancer treatments.
Keywords:
HepG2Long non-coding RNAgene expression profileginsenoside Rh2hepatocellular carcinoma

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