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Related Experiment Videos

Herpes simplex virus: recurrent and nonrecurrent strains.

Y Centifanto-Fitzgerald, D R Caldwell, F Yates

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)
    |September 1, 1987
    PubMed
    Summary

    Herpes Simplex Virus (HSV) strain type determines recurrent disease frequency and shedding. Temperature influences HSV reactivation, with non-recurrent strains failing to replicate at host core temperatures.

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    Area of Science:

    • Virology
    • Immunology
    • Ophthalmology

    Background:

    • Herpes Simplex Virus (HSV) causes recurrent disease.
    • Understanding HSV reactivation is crucial for disease management.
    • Viral and host factors influence HSV recurrence frequency.

    Purpose of the Study:

    • To investigate the relationship between HSV strain characteristics and recurrent disease frequency.
    • To explore the role of temperature in HSV reactivation and recurrence.
    • To differentiate mechanisms regulating HSV reactivation and disease.

    Main Methods:

    • Utilized the New Zealand white rabbit eye model to study three HSV strains (McKrae, E-43, CGA-3) with varying recurrence frequencies.
    • Assessed viral growth, disease manifestation, asymptomatic shedding, and iontophoretically induced recurrences.

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  • Investigated viral replication at different temperatures (33°C and 39°C) to understand temperature-dependency.

    • Main Results:

    • All three HSV strains caused initial corneal disease, but only McKrae and E-43 exhibited recurrences and shedding.
    • The non-recurrent CGA-3 strain failed to replicate at the host's core temperature (39°C) but replicated at 33°C, indicating temperature-dependent colonization and lack of recurrence.
    • Fresh clinical isolates from HSV encephalitis and herpes labialis showed preferential growth at 39°C.

    Conclusions:

    • HSV strain type is a key determinant of recurrent disease type (lesions, shedding, or none).
    • Viral reactivation and disease manifestation may be regulated by distinct mechanisms.
    • Host factors likely play a role in regulating the frequency of recurrent HSV disease.