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Expression Pattern of Cdkn2b and Its Regulators in Canine Mammary Tumors

Thamirys Aline Silva Faro1,2, Danilo DO Rosário Pinheiro1, Danielle Queiroz Calcagno3

  • 1Molecular Biology Laboratory, Biological Science Institute, Federal University of Pará, Belém, Brazil.

Anticancer Research
|November 7, 2018
PubMed
Abstract

Insights

Researchers investigated gene expression in canine mammary tumors. Key regulators like BMI1 proto-oncogene (Bmi1) and MYC proto-oncogene (Myc) showed increased expression, suggesting potential diagnostic value for veterinary cancer diagnostics.

Area of Science:

  • Veterinary Oncology
  • Molecular Biology
  • Canine Cancer Research

Background:

  • Mammary cancer is the most common tumor type in female dogs, representing 50% of all canine tumors.
  • The cell-cycle regulator cyclin-dependent kinase inhibitor 2B (Cdkn2b) is frequently altered in cancer.
  • Expression of Cdkn2b is negatively regulated by BMI1 proto-oncogene (Bmi1), MYC proto-oncogene (Myc), and T-box gene transcription factor 2 (Tbx2).

Purpose of the Study:

  • To evaluate the expression pattern of Cdkn2b and its regulators (Bmi1, Myc, Tbx2) in canine mammary tumors.
  • To investigate canine mammary tumors from female dogs in Northern Brazil (Belém, Pará).

Main Methods:

  • Gene expression analysis was performed using real-time polymerase chain reaction on samples from 33 animals.
  • Bisulfite sequencing polymerase chain reaction was employed to assess the influence of methylation on gene expression.

Main Results:

  • Increased expression levels of Bmi1, Myc, and Tbx2 were observed in tumor tissues compared to control samples.
  • Cdkn2b expression levels did not show a significant difference between tumor and control samples.
  • No correlation was found between gene expression and methylation data.

Conclusions:

  • The studied genes (Bmi1, Myc, Tbx2) may possess diagnostic value in veterinary oncology.
  • Further research is warranted to explore the role of these markers in canine mammary cancer.
Keywords:
Cell cyclecancerdogepigeneticgene regulation

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