Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

2.5K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
2.5K
Cell Adhesion Molecules - Types and Functions01:20

Cell Adhesion Molecules - Types and Functions

9.3K
Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily  involved...
9.3K
Cancers Originate from Somatic Mutations in a Single Cell02:21

Cancers Originate from Somatic Mutations in a Single Cell

14.9K
Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
14.9K
What are Cells?01:07

What are Cells?

199.6K
Cells are the smallest and basic units of life, whether it is a single cell that forms the entire organism, e.g., in a bacterium or trillions of them, e.g., in humans. No matter what organism a cell is a part of, they share specific characteristics.
Basic Characteristics of Cells
A living cell has a plasma membrane, a bilayer of lipids that separates the aqueous solution inside the cell called the cytoplasm from the outside environment.
Furthermore, a living cell possesses genetic information...
199.6K
Functional Groups02:45

Functional Groups

88.6K
Functional groups are a group of atoms with characteristic properties, which when linked to the carbon skeleton of a molecule, alter the properties of that molecule. For example, the presence of certain functional groups on a molecule will make them hydrophilic, whereas others will make them hydrophobic. These functional groups are an indispensable part of organic chemistry and important components of biological molecules, such as carbohydrates, proteins, lipids, and nucleic acids. Each...
88.6K
Chemistry of the Cell02:58

Chemistry of the Cell

48.0K
The cell is chemically composed of water, organic molecules and inorganic ions.
Water
The polarity of the water molecule and its resulting hydrogen bonding makes water a unique substance with special properties that are intimately tied to the processes of life. Life originally evolved in an aqueous environment, and most of an organism’s cellular chemistry and metabolism occur inside the aqueous contents of the cell’s cytoplasm. Special properties of water are its high heat capacity...
48.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Emerging Surgical Therapies for Vitiligo: Tissue vs Cell-Based Approaches.

Clinical, cosmetic and investigational dermatology·2026
Same author

Whole genome identification and operational analysis of the <i>bHLH</i> gene family in longan and the role of <i>DlbHLH8</i> in flowering regulation.

Frontiers in plant science·2026
Same author

In vitro expansion of stem-like memory T cells via D-mannose supplementation.

Methods in cell biology·2026
Same author

Correction: Association between Life's Essential 8 and psoriasis in US adults: a cross-sectional study.

Frontiers in medicine·2026
Same author

Decoding TCR specificity and T cell fate at the immunological synapse.

The Journal of experimental medicine·2026
Same author

Long&short Exposures Guided Diffusion Model for Realistic Local Motion Deblurring.

IEEE transactions on pattern analysis and machine intelligence·2026

Related Experiment Video

Updated: Feb 3, 2026

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms
06:50

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms

Published on: February 11, 2019

11.9K

Functional TCR T cell screening using single-cell droplet microfluidics.

Aude I Segaliny1, Guideng Li, Lingshun Kong

  • 1Sue and Bill Gross Stem Cell Research Center, Sue & Bill Gross Hall CIRM Institute, University of California, Irvine, 845 Health Sciences Road, Suite 3027, Irvine, CA 92697, USA. weianz@uci.edu.

Lab on a Chip
|November 7, 2018
PubMed
Summary
This summary is machine-generated.

Developing novel T cell therapies for cancer requires efficient screening. This study introduces a droplet microfluidics platform for rapid, multiplexed functional analysis and recovery of specific T cell receptor (TCR) T cell clones, accelerating cancer immunotherapy development.

More Related Videos

Droplet-based Cytotoxicity Assay to Assess Chimeric Antigen Receptor T cells at the Single-cell Level
08:09

Droplet-based Cytotoxicity Assay to Assess Chimeric Antigen Receptor T cells at the Single-cell Level

Published on: March 14, 2025

1.6K
An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
10:00

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

18.3K

Related Experiment Videos

Last Updated: Feb 3, 2026

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms
06:50

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms

Published on: February 11, 2019

11.9K
Droplet-based Cytotoxicity Assay to Assess Chimeric Antigen Receptor T cells at the Single-cell Level
08:09

Droplet-based Cytotoxicity Assay to Assess Chimeric Antigen Receptor T cells at the Single-cell Level

Published on: March 14, 2025

1.6K
An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
10:00

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

18.3K

Area of Science:

  • Immunology
  • Biotechnology
  • Oncology

Background:

  • Adoptive T cell transfer, particularly T cell receptor (TCR) T cell therapy, shows promise in cancer immunotherapy.
  • Identifying effective TCR T cell clones is currently a complex, time-intensive, and expensive challenge.
  • There is a significant need for advanced single-cell technologies to expedite functional analysis and clone recovery.

Purpose of the Study:

  • To develop and validate a droplet microfluidics platform for high-throughput functional screening of single T cells.
  • To enable real-time monitoring of T cell activation upon target recognition.
  • To facilitate the isolation and recovery of specific T cell clones for further therapeutic development.

Main Methods:

  • Utilized droplet microfluidics for the functional screening of single T cell receptor (TCR) T cells.
  • Implemented real-time monitoring of T cell activation against target tumor cells within droplets.
  • Integrated a clone tracking system and a high-specificity sorting procedure.
  • Validated clone recovery using single-cell reverse-transcription PCR and TCR sequencing.

Main Results:

  • Demonstrated a droplet microfluidics platform capable of functional screening and real-time monitoring of single T cell activation.
  • Achieved 100% specificity in sorting and recovery of T cell clones of interest.
  • Successfully validated recovered clones through downstream molecular analysis.

Conclusions:

  • The developed droplet microfluidics system significantly enhances the efficiency of screening T cell receptor (TCR) T cell clones.
  • This technology accelerates the identification and recovery of potent T cell clones for cancer immunotherapy.
  • The platform holds potential to streamline the development and clinical application of T cell-based therapies.