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Updated: Feb 3, 2026

Methylated DNA Immunoprecipitation
Published on: January 2, 2009
Quantification of DNA Methylation as Biomarker for Grain Quality
Christiane Seiler1, Markus Kuhlmann2
1Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany.
Abstract:
DNA methylation is an important biomarker for gene activity. It contributes to gene silencing and is involved in regulating various seed developmental processes in plants. Many of these processes are involved in important traits associated with aspects of grain quality. A reliable, fast, and cheap method is the estimation of DNA methylation utilizing methylation sensitive restriction enzymes (MSRE) and quantitative real-time PCR (qPCR) for selected candidate regions. The presented method can be used to confirm an effect of RNAi constructs on their target genes or trans-activity. Analysis of promoter regions can contribute to estimation of gene activity and related traits.
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