Related Experiment Video

Updated: Feb 3, 2026

Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans
04:51

Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans

Published on: November 14, 2016

9.7K

The Application of Recombinant Phototoxins 4D5scFv-miniSOG and DARPin-miniSOG to Study the HER2 Receptor

E O Kuzichkina1,2, O N Shilova3, S M Deyev3

  • 1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia. kuzichkinazhenya@mail.ru.

Doklady. Biochemistry and Biophysics
|November 7, 2018
PubMed

Insights

MiniSOG’s fluorescence helps track toxin binding and internalization in HER2-positive breast cancer cells. Cell fluorophores shield and absorb miniSOG fluorescence, explaining intensity decrease during toxin uptake.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Imaging

Background:

  • MiniSOG is a phototoxic protein with fluorescent properties.
  • HER2 receptor is a target in breast cancer therapy.
  • Understanding toxin-cell interactions is crucial for drug development.

Purpose of the Study:

  • To evaluate miniSOG’s utility in assessing toxin binding to SK-BR-3 cells.
  • To investigate the dynamics of recombinant protein internalization.
  • To elucidate the cause of fluorescence intensity decrease during cellular uptake.

Main Methods:

  • Utilized the fluorescent properties of the miniSOG phototoxic domain.
  • Assessed binding of recombinant proteins (4D5scFv-miniSOG, DARPin-miniSOG) to SK-BR-3 cells.
  • Studied the dynamics of protein internalization via fluorescence microscopy.

Main Results:

  • MiniSOG fluorescence effectively monitors toxin binding and internalization in SK-BR-3 cells.
  • Recombinant proteins showed internalization in complex with the HER2 receptor.
  • Fluorescence intensity decreased due to shielding and absorption by cellular fluorophores.

Conclusions:

  • MiniSOG is a valuable tool for studying molecular interactions with cancer cells.
  • Cellular microenvironment significantly impacts miniSOG fluorescence during internalization.
  • This study provides insights into the mechanisms of targeted toxin delivery.

More Related Videos

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging ESI13:06

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging ESI

Published on: September 24, 2015

10.5K
A Cell Free Assay System Estimating the Neutralizing Capacity of GM-CSF Antibody using Recombinant Soluble GM-CSF Receptor07:45

A Cell Free Assay System Estimating the Neutralizing Capacity of GM-CSF Antibody using Recombinant Soluble GM-CSF Receptor

Published on: June 27, 2011

13.0K

Related Concept Videos

Internal Receptors01:31

Internal Receptors

Many cellular signals are hydrophilic and therefore cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind to internal, or intracellular, receptors that reside within the cell. Many mammalian steroid hormones use this mechanism of cell signaling, as does nitric oxide (NO) gas.
74.7K
Types of Receptors: Internal Receptors01:07

Types of Receptors: Internal Receptors

Many cellular signals are hydrophilic and cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind intracellular receptors that reside within the cell cytoplasm or nucleus. Many mammalian steroid hormones and nitric oxide (NO) gas use this cell signaling mechanism.
Similar to membrane-bound receptors, the binding of a ligand to the intracellular receptor of causes a conformational change in the...
32.6K
Recombinant DNA01:09

Recombinant DNA

Overview
103.0K
Internal Energy02:00

Internal Energy

The total of all possible kinds of energy present in a substance is called the internal energy (U), sometimes symbolized as E. Suppose a system with initial internal energy, Uinitial, undergoes a change in energy (transfer of work or heat), and the final internal energy of the system is Ufinal. Change in internal energy equals the difference between Ufinal and Uinitial.
36.8K
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
19.3K
Viral Recombination00:57

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
25.1K