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Hemagglutination with transmissible gastroenteritis virus.

M Noda, H Yamashita, F Koide

    Archives of Virology
    |January 1, 1987
    PubMed
    Summary
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    Transmissible gastroenteritis virus causes swine disease and agglutinates certain red blood cells. Hemagglutination inhibition antibody titers correlate with neutralizing antibody titers in pigs.

    Area of Science:

    • Veterinary Virology
    • Immunology

    Background:

    • Transmissible gastroenteritis virus (TGEV) is a significant pathogen in swine.
    • Understanding TGEV's interaction with host cells is crucial for disease control.

    Purpose of the Study:

    • To investigate the hemagglutination (HA) properties of TGEV.
    • To establish standard hemagglutination inhibition (HI) tests for TGEV.
    • To correlate HI antibody titers with neutralizing antibody titers in pigs.

    Main Methods:

    • TGEV was propagated in primary swine kidney cell cultures.
    • Hemagglutination assays were performed using erythrocytes from various animal species.
    • Hemagglutination inhibition tests were conducted using specific antisera.
    • Antibody titers in pig sera were determined using both HI and neutralization assays.

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    Main Results:

    • TGEV agglutinated erythrocytes from chickens, guinea pigs, and cattle, but not mice or geese.
    • Optimal agglutination occurred at 4 degrees C.
    • Specific antiserum inhibited the HA reaction.
    • Established standard HA and HI tests.
    • A significant positive correlation was observed between HI and neutralizing antibody titers in pig sera.

    Conclusions:

    • TGEV exhibits hemagglutinating activity, suggesting its potential use in diagnostic assays.
    • Standardized HA and HI tests can be reliably used for TGEV antibody detection.
    • HI antibody titers serve as a reliable indicator of neutralizing antibody levels against TGEV in pigs.