Related Experiment Video
Updated: Feb 3, 2026

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry
Published on: June 8, 2012
The multi-spectral imaging diagnostic
B L Linehan1, R T Mumgaard1, M Wensing2
1Plasma Science and Fusion Center MIT, Cambridge, Massachusetts 02139, USA.
A novel Multi-Spectral Imaging (MSI) system captures simultaneous atomic line intensity images. This diagnostic enables 2D mappings of atomic line ratios and absolute intensities for advanced plasma diagnostics.
Area of Science:
- Plasma physics
- Optical diagnostics
- Spectroscopy
Background:
- Advanced diagnostics are crucial for understanding fusion plasmas.
- Existing imaging systems face limitations in throughput and spectral resolution.
- Multi-spectral imaging offers potential for simultaneous multi-wavelength measurements.
Purpose of the Study:
- To introduce and validate a novel Multi-Spectral Imaging (MSI) system for plasma diagnostics.
- To demonstrate the capability of MSI for 2D mapping of atomic line intensities and ratios.
- To assess the performance of the MSI system in terms of resolution, calibration, and throughput.
Main Methods:
- A modified polychromator design enables sequential filtering and focusing of images.
- Commercial CMOS cameras are utilized for image acquisition.
- Absolute calibration is performed using an integrating sphere and cross-referencing with CAD models.
- The system was tested on Alcator C-Mod and TCV tokamak devices.
Main Results:
- The MSI system successfully captured simultaneous spectrally filtered images.
- 2D mappings of atomic line ratios and absolute intensities were achieved with high spatial registration.
- Minimal vignetting and aberrations were observed.
- The system demonstrated high throughput and accurate spectral transmission calibration.
Conclusions:
- The developed Multi-Spectral Imaging system is a viable and effective diagnostic tool for fusion research.
- MSI enables advanced plasma characterization through simultaneous multi-wavelength imaging.
- The system's calibration and registration capabilities allow for quantitative analysis of plasma properties.
Related Concept Videos
Multi-input and Multi-variable systems
In the absence of...
Appendicitis-II: Diagnostic Studies and Management
Diagnosing Appendicitis
It requires a multifaceted approach, starting with a detailed physical examination to pinpoint the location and nature of the pain and identify any associated symptoms. Laboratory tests play a crucial role. A complete Blood Count (CBC) typically reveals leukocytosis (an increased number of...
Myasthenia Gravis: Diagnostic Tests
The edrophonium test is a diagnostic tool for myasthenia gravis. It involves...
Asthma-IV: Diagnostic and Management
Clinical Assessment for Asthma:
This is the first step in diagnosing and managing asthma. It includes:
Pericarditis II: Clinical Features and Diagnostic Tests
Atherosclerosis II: Clinical Manifestations and Diagnostic Tests

